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Published Online February 12, 2009
Science DOI: 10.1126/science.1167373

Research Articles

Submitted on October 20, 2008
Accepted on January 27, 2009

The Dynamic Control of Kiss-And-Run and Vesicular Reuse Probed with Single Nanoparticles

Qi Zhang 1, Yulong Li 1, Richard W. Tsien 1*

1 Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA.

* To whom correspondence should be addressed.
Richard W. Tsien , E-mail: rwtsien{at}stanford.edu

Vesicular secretion of neurotransmitter is essential for neuronal communication. Kiss-and-run is a mode of membrane fusion and retrieval without the full collapse of the vesicle into the plasma membrane and de novo regeneration. The significance of kiss-and-run during efficient neurotransmission has remained in doubt. We developed an approach for loading individual synaptic vesicles with single quantum dots. Their size and pH-dependent photoluminescence change allowed us to distinguish kiss-and-run from full-collapse fusion and to track single vesicles through multiple rounds of kiss-and-run and reuse, without perturbing vesicle cycling. Kiss-and-run dominated at the beginning of stimulus trains, reflecting the preference of vesicles with high release probability. Its incidence was increased by rapid firing, a response appropriate to shape the kinetics of neurotransmission during a wide range of firing patterns.


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