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Submitted on November 14, 2007
Accepted on January 3, 2008
Differential Regulation of Dynein and Kinesin Motor Proteins by Tau
Ram Dixit 1,Jennifer L. Ross 2,Yale E. Goldman 1,Erika L. F. Holzbaur 1*
1 Department of Physiology and Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, PA 19104. USA. 2 Department of Physiology and Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, PA 19104. USA.; Present address: 302 Hasbrouck Laboratory, Department of Physics, University of Massachusetts at Amherst, Amherst, MA
01003. USA.
* To whom correspondence should be addressed.
Erika L. F. Holzbaur , E-mail: holzbaur{at}mail.med.upenn.edu
Dynein and kinesin motor proteins transport cellular cargostoward opposite ends of microtubule tracks. In neurons, microtubulesare abundantly decorated with microtubule-associated proteins(MAPs) such as tau. Motor proteins thus encounter MAPs frequentlyalong their path. To determine the effects of tau on dyneinand kinesin motility, we conducted single molecule studies ofmotor proteins moving along tau-decorated microtubules. Dyneintended to reverse direction whereas kinesin tended to detachat patches of bound tau. Kinesin was inhibited at ~10-fold lowertau concentration than dynein and the microtubule-binding domainof tau was sufficient to inhibit motor activity. The differentialmodulation of dynein and kinesin motility suggests that MAPscan spatially regulate the balance of microtubule-dependentaxonal transport.
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