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Published Online February 7, 2008
Science DOI: 10.1126/science.1151461

Reports

Submitted on October 9, 2007
Accepted on January 21, 2008

TOPLESS Mediates Auxin-Dependent Transcriptional Repression During Arabidopsis Embryogenesis

Heidi Szemenyei 1, Mike Hannon 1, Jeff A. Long 2*

1 Plant Biology Laboratory, The Salk Institute for Biological Sciences, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.; Division of Biological Sciences, Section of Cell and Developmental Biology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
2 Plant Biology Laboratory, The Salk Institute for Biological Sciences, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.

* To whom correspondence should be addressed.
Jeff A. Long , E-mail: long{at}salk.edu

The transcriptional response to auxin is critical for root and vascular development during Arabidopsis embryogenesis. Auxin induces the degradation of AUX/IAA transcriptional repressors, freeing their binding partners, the AUXIN RESPONSE FACTOR (ARF) proteins, which can activate transcription of auxin response genes. Here we show that TOPLESS (TPL) can physically interact with IAA12/BODENLOS (IAA12/BDL) through an ERF-associated amphiphilic repression (EAR) motif. TPL can repress transcription in vivo and is required for IAA12/BDL repressive activity. In addition, tpl-1 can suppress the patterning defects of the bdl-1 mutant. Direct interaction between TPL and ARF5/MONOPTEROS, which is regulated by IAA12/BDL, results in a loss of function arf5/mp phenotype. These observations show that TPL is a transcriptional corepressor and further our understanding of how auxin regulates transcription during plant development.


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