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Published Online September 13, 2007
Science DOI: 10.1126/science.1146994

Reports

Submitted on June 25, 2007
Accepted on August 28, 2007

FKF1 and GIGANTEA Complex Formation is Required for Day-Length Measurement in Arabidopsis

Mariko Sawa 1, Dmitri A. Nusinow 1, Steve A. Kay 1, Takato Imaizumi 1*

1 Department of Biochemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

* To whom correspondence should be addressed.
Takato Imaizumi , E-mail: takato{at}scripps.edu

Precise timing of CONSTANS (CO) gene expression is necessary for day-length discrimination for photoperiodic flowering. The FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 (FKF1) and GIGANTEA (GI) proteins regulate CO transcription in Arabidopsis. We demonstrate that FKF1 and GI proteins form a complex in a blue-light dependent manner. The timing of this interaction regulates the timing of daytime CO expression. FKF1 function is dependent on GI, which interacts with a CO repressor, CYCLING DOF FACTOR 1 (CDF1), and controls CDF1 stability. GI, FKF1, and CDF1 proteins associate with CO chromatin. Thus, the FKF1-GI complex forms on the CO promoter in late afternoon to regulate CO expression, providing a mechanistic view of how the coincidence of light with circadian timing regulates photoperiodic flowering.


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