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Published Online June 7, 2007
Science DOI: 10.1126/science.1143422

Reports

Submitted on April 4, 2007
Accepted on May 25, 2007

Parallels Between Cytokinesis and Retroviral Budding: A Role for the ESCRT Machinery

Jez G. Carlton 1 and Juan Martin-Serrano 1*

1 Department of Infectious Diseases, King's College London School of Medicine, London, UK.

* To whom correspondence should be addressed.
Juan Martin-Serrano , E-mail: juan.martin_serrano{at}kcl.ac.uk

During cytokinesis, as dividing animal cells pull apart into two daughter cells, the final stage, termed abscission, requires breakage of the midbody, a thin membranous stalk connecting the daughter cells. This membrane fission event topologically resembles budding of viruses, such as HIV-1 from infected cells. Here we found that two proteins involved in HIV-1 budding--Tsg101, a subunit of the Endosomal Sorting Complex Required for Transport-I (ESCRT-I) and Alix, an ESCRT-associated protein--were recruited to the midbody during cytokinesis by interaction with Cep55, a centrosome and midbody-protein essential for abscission. Tsg101, Alix and possibly other components of ESCRT-I were required for completion of cytokinesis. Thus HIV-1 budding and cytokinesis utilize a similar subset of cellular components to carry out topologically similar membrane fission events.


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