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Published Online April 5, 2007
Science DOI: 10.1126/science.1141314

Reports

Submitted on February 14, 2007
Accepted on March 23, 2007

Genes Required for Mitotic Spindle Assembly in Drosophila S2 Cells

Gohta Goshima 1, Roy Wollman 2, Sarah S. Goodwin 3, Nan Zhang 3, Jonathan M. Scholey 4, Ronald D. Vale 5*, Nico Stuurman 5

1 The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA; The Physiology Course, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543, USA; Present address: Institute for Advanced Research, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan.
2 Department of Molecular and Cellular Biology, University of California, Davis, One Shields Avenue, 3203 Life Sciences, Davis, CA 95616, USA; The Physiology Course, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543, USA.
3 The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
4 Department of Molecular and Cellular Biology, University of California, Davis, One Shields Avenue, 3203 Life Sciences, Davis, CA 95616, USA.
5 The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA; The Physiology Course, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543, USA.

* To whom correspondence should be addressed.
Ronald D. Vale , E-mail: vale{at}cmp.ucsf.edu

The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full genome RNAi screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.


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