Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Submitted on December 15, 2006
Accepted on May 7, 2007
Genome Sequence of Aedes aegypti, a Major Arbovirus Vector
Vishvanath Nene 1*, Jennifer R. Wortman 1, Daniel Lawson 2, Brian Haas 1, Chinnappa Kodira 3, Zhijian Jake Tu 4, Brendan Loftus 5, Zhijong Xi 6, Karyn Megy 2, Manfred Grabherr 3, Quinghu Ren 1, Evgeny M. Zdobnov 7, Neil F. Lobo 8, Kathryn S. Campbell 9, Susan E. Brown 10, Maria F. Bonaldo 11, Jingsong Zhu 12, Steven P. Sinkins 13, David G. Hogenkamp 14, Paulo Amedo 1, Peter Arsenburger 12, Peter W. Atkinson 12, Shelby Bidwell 1, Jim Biedler 4, Ewan Birney 2, Robert V. Bruggner 8, Javier Costas 15, Monique R. Coy 4, Jonathan Crabtree 1, Matt Crawford 3, Becky deBruyn 8, David DeCaprio 3, Karin Eiglmeier 16, Eric Eisenstadt 1, Hamza El-Dorry 17, William M. Gelbart 9, Suely L. Gomes 17, Martin Hammond 2, Linda I. Hannick 1, James R. Hogan 8, Michael H. Holmes 1, David Jaffe 3, Spencer J. Johnston 18, Ryan C. Kennedy 8, Hean Koo 1, Saul Kravitz 19, Evgenia V. Kriventseva 20, David Kulp 21, Kurt LaButti 3, Edward Lee 1, Song Li 4, Diane D. Lovin 8, Chunhong Mao 4, Evan Mauceli 3, Carlos F. M. Menck 22, Jason R. Miller 1, Philip Montgomery 3, Akio Mori 8, Ana L. Nascimento 23, Horacio F. Naveira 24, Chad Nusbaum 3, Sinéad B. O’Leary 3, Joshua Orvis 1, Mihaela Pertea 25, Hadi Quesneville 26, Kyanne R. Reidenbach 14, Yu-Hui Rogers 19, Charles W. Roth 16, Jennifer R. Schneider 8, Michael Schatz 25, Martin Shumway 1, Mario Stanke 27, Eric O. Stinson 8, Jose M. C. Tubio 28, Janice P. VanZee 14, Sergio Verjovski-Almeida 17, Doreen Werner 29, Owen White 1, Stefan Wyder 20, Qi Zeng 3, Qi Zhao 1, Yongmei Zhao 1, Catherine A. Hill 14, Alexander S. Raikhel 12, Marcelo B. Soares 11, Dennis L. Knudson 10, Norman H. Lee 30, James Galagan 3, Steven L. Salzberg 25, Ian T. Paulsen 1, George Dimopoulos 6, Frank H. Collins 8, Birren Bruce 3, Claire M. Fraser-Liggett 1, David W. Severson 8*
1 The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA. 2 European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SD, UK. 3 Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02141, USA. 4 Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. 5 The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.; Present address: University College Dublin, Dublin 4, Ireland. 6 Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA. 7 University of Geneva Medical School, 1 rue Michel-Servet, Geneva 1211, Switzerland.; Swiss Institute of Bioinformatics, 1 rue Michel-Servet, Geneva 1211, Switzerland.; Imperial College London, South Kensington Campus, London SW7 2AZ, UK. 8 University of Notre Dame, Notre Dame, IN 46556, USA. 9 Harvard University, Cambridge, MA 02138, USA. 10 College of Agricultural Sciences, Colorado State University, Fort Collins, CO 80523, USA. 11 Northwestern University, Chicago, IL 60614, USA. 12 University of California, Riverside, CA 92521, USA. 13 University of Oxford, Oxford OX1 3PS, UK. 14 Purdue University, West Lafayette, IN 47907, USA. 15 Centro Nacional de Genotipado, Fundación Pública Galega de Medicina Xenómica, Hospital Clínico Universitario de Santiago, Edif. Consultas Planta -2, Santiago de Compostela E-15706, Spain. 16 Institut Pasteur, Paris 75724, France. 17 Universidade de Sao Paulo, Instituto de Quimica, Sao Paulo SP 05508-900, Brazil. 18 Texas A&M University, College Station, TX 77843, USA. 19 Joint Technology Center, 5 Research Place, Rockville, MD 20850, USA. 20 University of Geneva Medical School, 1 rue Michel-Servet, Geneva 1211, Switzerland. 21 University of Massachusetts, Amherst, MA 01003, USA. 22 Universidade de Sao Paulo, Institute of Biomedical Sciences, Sao Paulo SP 05508-900, Brazil. 23 Instituto Butantan, Sao Paulo SP 05503-900, Brazil. 24 Universidade da Coruña, 15001 A Coruña, Spain. 25 The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.; Present address: 3125 Biomolecular Sciences Building, University of Maryland, College Park, MD 20742, USA. 26 Institut Jacques Monod, CNRS, Université Paris Diderot et Université Pierre-et-Marie Curie 2, Place Jussieu, Paris 75252, France. 27 507A Engineering 2, University of California, 1156 High Street, Santa Cruz, CA 95064, USA.; Universität Göttingen, Goldschmidtstraße 1, Göttingen 37077, Germany. 28 Complexo Hospitalario Universitario de Santiago, Santiago de Compostela 15706, Spain. 29 Universität Göttingen, Goldschmidtstraße 1, Göttingen 37077, Germany. 30 The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.; Present address: George Washington University Medical Center, Ross Hall, Room 603, 2300 I Street NW, Washington, DC 20037, USA.
* To whom correspondence should be addressed.
Vishvanath Nene , E-mail: nene{at}tigr.org David W. Severson , E-mail: severson.1{at}nd.edu
We present a draft sequence of the genome of Aedes aegypti,the primary vector for yellow fever and dengue fever, whichat ~1.38 Gbp is ~5-fold larger in size than the genome of themalaria vector, Anopheles gambiae. Nearly 50% of the Aedes aegyptigenome consists of transposable elements. These contribute toa ~4-6 fold increase in average gene length and the size ofintergenic regions relative to Anopheles gambiae and Drosophilamelanogaster. Nevertheless, chromosomal synteny is generallymaintained between all three insects although conservation oforthologous gene order is higher (~2-fold) between the mosquitospecies than between either of them and fruit fly. An increasein genes encoding odorant binding, cytochrome P450 and cuticledomains relative to Anopheles gambiae suggests that membersof these protein families underpin some of the biological differencesbetween them.
The editors suggest the following Related Resources on Science sites:
In Science Magazine
PERSPECTIVES
Dave D. Chadee, Pattamaporn Kittayapong, Amy C. Morrison, and Walter J. Tabachnick (22 June 2007) Science316 (5832), 1703.
[DOI: 10.1126/science.1138904] |Summary »|Full Text »|PDF »
REPORTS
Robert M. Waterhouse, Evgenia V. Kriventseva, Stephan Meister, Zhiyong Xi, Kanwal S. Alvarez, Lyric C. Bartholomay, Carolina Barillas-Mury, Guowu Bian, Stephanie Blandin, Bruce M. Christensen, Yuemei Dong, Haobo Jiang, Michael R. Kanost, Anastasios C. Koutsos, Elena A. Levashina, Jianyong Li, Petros Ligoxygakis, Robert M. MacCallum, George F. Mayhew, Antonio Mendes, Kristin Michel, Mike A. Osta, Susan Paskewitz, Sang Woon Shin, Dina Vlachou, Lihui Wang, Weiqi Wei, Liangbiao Zheng, Zhen Zou, David W. Severson, Alexander S. Raikhel, Fotis C. Kafatos, George Dimopoulos, Evgeny M. Zdobnov, and George K. Christophides (22 June 2007) Science316 (5832), 1738.
[DOI: 10.1126/science.1139862] |Abstract »|Full Text »|PDF »|Supporting Online Material »
In Science Signaling
EDITORS' CHOICE
Barbara R. Jasny (26 June 2007) Sci. STKE2007 (392), tw229.
[DOI: 10.1126/stke.3922007tw229] |Abstract »
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Chromosomal Rearrangement Inferred From Comparisons of 12 Drosophila Genomes.
A. Bhutkar, S. W. Schaeffer, S. M. Russo, M. Xu, T. F. Smith, and W. M. Gelbart (2008)
Genetics
179, 1657-1680
|Abstract »|Full Text »|PDF »
Pirk Is a Negative Regulator of the Drosophila Imd Pathway.
A. Kleino, H. Myllymaki, J. Kallio, L.-M. Vanha-aho, K. Oksanen, J. Ulvila, D. Hultmark, S. Valanne, and M. Ramet (2008)
J. Immunol.
180, 5413-5422
|Abstract »|Full Text »|PDF »
Genetic Dissociation of Ethanol Sensitivity and Memory Formation in Drosophila melanogaster.
H. LaFerriere, D. J. Guarnieri, D. Sitaraman, S. Diegelmann, U. Heberlein, and T. Zars (2008)
Genetics
178, 1895-1902
|Abstract »|Full Text »|PDF »
Cloning and identification of an oxytocin/vasopressin-like receptor and its ligand from insects.
E. Stafflinger, K. K. Hansen, F. Hauser, M. Schneider, G. Cazzamali, M. Williamson, and C. J. P. Grimmelikhuijzen (2008)
PNAS
105, 3262-3267
|Abstract »|Full Text »|PDF »
The Gr Family of Candidate Gustatory and Olfactory Receptors in the Yellow-Fever Mosquito Aedes aegypti.
L. B. Kent, K. K.O. Walden, and H. M. Robertson (2008)
Chem Senses
33, 79-93
|Abstract »|Full Text »|PDF »
Noduler, A Novel Immune Up-Regulated Protein Mediates Nodulation Response in Insects.
A. S. Gandhe, S. H. John, and J. Nagaraju (2007)
J. Immunol.
179, 6943-6951
|Abstract »|Full Text »|PDF »
Developmental genomics of the most dangerous animal.
Evolutionary Dynamics of Immune-Related Genes and Pathways in Disease-Vector Mosquitoes.
R. M. Waterhouse, E. V. Kriventseva, S. Meister, Z. Xi, K. S. Alvarez, L. C. Bartholomay, C. Barillas-Mury, G. Bian, S. Blandin, B. M. Christensen, et al. (2007)
Science
316, 1738-1743
|Abstract »|Full Text »|PDF »
nanos gene control DNA mediates developmentally regulated transposition in the yellow fever mosquito Aedes aegypti.
Z. N. Adelman, N. Jasinskiene, S. Onal, J. Juhn, A. Ashikyan, M. Salampessy, T. MacCauley, and A. A. James (2007)
PNAS
104, 9970-9975
|Abstract »|Full Text »|PDF »
