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Submitted on October 3, 2006
Accepted on January 24, 2007
A G Protein-Coupled Receptor Is a Plasma Membrane Receptor for the Plant Hormone Abscisic Acid
Xigang Liu 1, Yanling Yue 2, Bin Li 3, Yanli Nie 2, Wei Li 4, Wei-Hua Wu 3, Ligeng Ma 1*
1 National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing 102206, China; Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, Hebei 050016, China. 2 National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing 102206, China. 3 State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China. 4 Laboratory of Molecular and Cellular Biology, Hebei Normal University, Shijiazhuang, Hebei 050016, China.
* To whom correspondence should be addressed.
Ligeng Ma , E-mail: maligeng{at}nibs.ac.cn
The plant hormone abscisic acid (ABA) regulates many physiologicaland developmental processes in plants. The mechanism of ABAperception at cell surface is not understood. Here we show thata G protein-coupled receptor genetically and physically interactswith the GPA1 to mediate all known ABA responses in Arabidopsis.Overexpressing this receptor results in an ABA-hypersensitivephenotype. This receptor binds ABA with high affinity at physiologicalconcentration with expected kinetics and stereospecificity.The binding of ABA to the receptor leads to the dissociationof the receptor-GPA1 complex in yeast. Our results demonstratethat this G protein-coupled receptor is a plasma membrane ABAreceptor.
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