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Published Online December 14, 2006
Science DOI: 10.1126/science.1133542

Research Articles

Submitted on August 7, 2006
Accepted on December 6, 2006

Histocompatible Embryonic Stem Cells by Parthenogenesis

Kitai Kim 1, Paul Lerou 2, Akiko Yabuuchi 1, Claudia Lengerke 1, Kitwa Ng 1, Jason West 1, Andrew Kirby 3, Mark J. Daly 3, George Q. Daley 4*

1 Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA.
2 Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Division of Newborn Medicine, Brigham and Women's Hospital and Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
3 Center for Human Genetic Research, Massachusetts General Hospital, Boston, MA 02115, USA.
4 Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA; Division of Hematology, Brigham and Women's Hospital, Boston, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA.

* To whom correspondence should be addressed.
George Q. Daley , E-mail: george.daley{at}childrens.harvard.edu

Genetically matched pluripotent embryonic stem cells generated via nuclear transfer (ntES cells) or parthenogenesis (pES cells) are a potential source of histocompatible cells and tissues for transplantation. Following parthenogenetic activation of murine oocytes and interruption of meiosis I or II, we have isolated and genotyped pES cells and characterized those that carry the full complement of major histocompatibility complex (MHC) antigens of the oocyte donor. Differentiated tissues from these pES cells engraft in immunocompetent MHC-matched mouse recipients, demonstrating that selected pES cells can serve as a source of histocompatible tissues for transplantation.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)