Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Published Online November 9, 2006
Science DOI: 10.1126/science.1133141

Reports

Submitted on July 28, 2006
Accepted on November 2, 2006

A Complex Oscillating Network of Signaling Genes Underlies the Mouse Segmentation Clock

Mary-Lee Dequéant 1, Earl Glynn 2, Karin Gaudenz 2, Matthias Wahl 2, Jie Chen 3, Arcady Mushegian 2, Olivier Pourquié 4*

1 Stowers Institute for Medical Research, Kansas City, MO 64110, USA; University of Kansas Medical Center, Kansas City, KS 66160, USA.
2 Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
3 Stowers Institute for Medical Research, Kansas City, MO 64110, USA; University of Missouri-Kansas City, MO, 64110, USA.
4 Stowers Institute for Medical Research, Kansas City, MO 64110, USA; Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.

* To whom correspondence should be addressed.
Olivier Pourquié , E-mail: olp{at}stowers-institute.org

The segmental pattern of the spine is established early in development when the vertebral precursors, the somites, are rhythmically produced from the presomitic mesoderm. Microarray studies of the mouse presomitic mesoderm transcriptome reveal that the oscillator associated with this process-the segmentation clock-drives the periodic expression of a large network of cyclic genes involved in cell signaling. Mutually exclusive activation of the Notch/FGF and Wnt pathways during each cycle, suggests that coordinated regulation of these three pathways underlies the clock oscillator.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Pushing the frontiers of development.
Y. Bellaiche and E. Munro (2009)
Development 136, 173-177
   Abstract »    Full Text »    PDF »
Mutation of HAIRY-AND-ENHANCER-OF-SPLIT-7 in humans causes spondylocostal dysostosis.
D. B. Sparrow, E. Guillen-Navarro, D. Fatkin, and S. L. Dunwoodie (2008)
Hum. Mol. Genet. 17, 3761-3766
   Abstract »    Full Text »    PDF »
Anterior-posterior patterning and segmentation of the vertebrate head.
T. F. Schilling (2008)
Integr. Comp. Biol. 48, 658-667
   Abstract »    Full Text »    PDF »
The molecular ancestry of segmentation mechanisms.
E. M. De Robertis (2008)
PNAS 105, 16411-16412
   Full Text »    PDF »
From Signals to Patterns: Space, Time, and Mathematics in Developmental Biology.
J. Lewis (2008)
Science 322, 399-403
   Abstract »    Full Text »    PDF »
Whole-Mount In Situ Hybridization in Monodelphis Embryos.
A. L. Keyte and K. K. Smith (2008)
CSH Protocols 2008, pdb.prot5076
   Abstract »    Full Text »
Noncyclic Notch activity in the presomitic mesoderm demonstrates uncoupling of somite compartmentalization and boundary formation.
J. Feller, A. Schneider, K. Schuster-Gossler, and A. Gossler (2008)
Genes & Dev. 22, 2166-2171
   Abstract »    Full Text »    PDF »
Cell cycle progression is required for zebrafish somite morphogenesis but not segmentation clock function.
L. Zhang, C. Kendrick, D. Julich, and S. A. Holley (2008)
Development 135, 2065-2070
   Abstract »    Full Text »    PDF »
Oscillatory lunatic fringe activity is crucial for segmentation of the anterior but not posterior skeleton.
E. T. Shifley, K. M. VanHorn, A. Perez-Balaguer, J. D. Franklin, M. Weinstein, and S. E. Cole (2008)
Development 135, 899-908
   Abstract »    Full Text »    PDF »
Wnt/Notch signalling and information processing during development.
P. Hayward, T. Kalmar, and A. Martinez Arias (2008)
Development 135, 411-424
   Abstract »    Full Text »    PDF »
Wnt3a/ -catenin signaling controls posterior body development by coordinating mesoderm formation and segmentation.
W. C. Dunty Jr, K. K. Biris, R. B. Chalamalasetty, M. M. Taketo, M. Lewandoski, and T. P. Yamaguchi (2008)
Development 135, 85-94
   Abstract »    Full Text »    PDF »
FGF signaling acts upstream of the NOTCH and WNT signaling pathways to control segmentation clock oscillations in mouse somitogenesis.
M. B. Wahl, C. Deng, M. Lewandoski, and O. Pourquie (2007)
Development 134, 4033-4041
   Abstract »    Full Text »    PDF »
A High-Resolution Root Spatiotemporal Map Reveals Dominant Expression Patterns.
S. M. Brady, D. A. Orlando, J.-Y. Lee, J. Y. Wang, J. Koch, J. R. Dinneny, D. Mace, U. Ohler, and P. N. Benfey (2007)
Science 318, 801-806
   Abstract »    Full Text »    PDF »
High-resolution timing of cell cycle-regulated gene expression.
M. Rowicka, A. Kudlicki, B. P. Tu, and Z. Otwinowski (2007)
PNAS 104, 16892-16897
   Abstract »    Full Text »    PDF »
Watch-ing out for chick limb development.
S. Pascoal and I. Palmeirim (2007)
Integr. Comp. Biol. 47, 382-389
   Abstract »    Full Text »    PDF »
Viable Mice with Compound Mutations in the Wnt/Dvl Pathway Antagonists nkd1 and nkd2.
S. Zhang, T. Cagatay, M. Amanai, M. Zhang, J. Kline, D. H. Castrillon, R. Ashfaq, O. K. Oz, and K. A. Wharton Jr. (2007)
Mol. Cell. Biol. 27, 4454-4464
   Abstract »    Full Text »    PDF »
Building the Spine: The Vertebrate Segmentation Clock.
O. Pourquie (2007)
Cold Spring Harb Symp Quant Biol 72, 445-449
   Abstract »    PDF »
Ultradian Oscillators in Somite Segmentation and Other Biological Events.
R. Kageyama, S. Yoshiura, Y. Masamizu, and Y. Niwa (2007)
Cold Spring Harb Symp Quant Biol 72, 451-457
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)