Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Published Online November 16, 2006
Science DOI: 10.1126/science.1132397

Reports

Submitted on July 12, 2006
Accepted on November 7, 2006

A Gain-of-Function Mutation in a Cytokinin Receptor Triggers Spontaneous Root Nodule Organogenesis

Leïla Tirichine 1, Niels Sandal 1, Lene H. Madsen 1, Simona Radutoiu 1, Anita S. Albrektsen 1, Shusei Sato 2, Erika Asamizu 2, Satoshi Tabata 2, Jens Stougaard 1*

1 Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark.
2 Kazusa DNA Research Institute, Kisarazu, Chiba, 292-0818, Japan.

* To whom correspondence should be addressed.
Jens Stougaard , E-mail: stougaard{at}mb.au.dk

Legume root nodules originate from differentiated cortical cells that reenter the cell cycle and form organ primordia. We show that perception of the phytohormone cytokinin is a key element in this switch. Mutation of a Lotus japonicus cytokinin receptor gene leads to spontaneous development of root nodules in the absence of rhizobia or rhizobial signal molecules. The mutant histidine kinase receptor has cytokinin-independent activity and activates an Escherichia coli two-component phosphorelay system in vivo. Mutant analysis shows that cytokinin signaling is required for cell divisions initiating nodule development and defines an autoregulated process where cytokinin induction of nodule stem cells is shoot controlled.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A Small GTPase of the Rab Family Is Required for Root Hair Formation and Preinfection Stages of the Common Bean-Rhizobium Symbiotic Association.
F. A. Blanco, E. Peltzer Meschini, M. E. Zanetti, and O. M. Aguilar (2009)
PLANT CELL 21, 2797-2810
   Abstract »    Full Text »    PDF »
A Genome-Wide Compilation of the Two-Component Systems in Lotus japonicus.
K. Ishida, Y. Niwa, T. Yamashino, and T. Mizuno (2009)
DNA Res 16, 237-247
   Abstract »    Full Text »    PDF »
Symbiotic and Growth Performance of Supernodulating Forage Pea Lines.
K. Novak, E. Biedermannova, and J. Vondrys (2009)
Crop Sci. 49, 1227-1234
   Abstract »    Full Text »    PDF »
Calcium Spiking Patterns and the Role of the Calcium/Calmodulin-Dependent Kinase CCaMK in Lateral Root Base Nodulation of Sesbania rostrata.
W. Capoen, J. Den Herder, J. Sun, C. Verplancke, A. De Keyser, R. De Rycke, S. Goormachtig, G. Oldroyd, and M. Holsters (2009)
PLANT CELL 21, 1526-1540
   Abstract »    Full Text »    PDF »
The Temperature-Sensitive brush Mutant of the Legume Lotus japonicus Reveals a Link between Root Development and Nodule Infection by Rhizobia.
M. Maekawa-Yoshikawa, J. Muller, N. Takeda, T. Maekawa, S. Sato, S. Tabata, J. Perry, T. L. Wang, M. Groth, A. Brachmann, et al. (2009)
Plant Physiology 149, 1785-1796
   Abstract »    Full Text »    PDF »
Roles for Auxin, Cytokinin, and Strigolactone in Regulating Shoot Branching.
B. J. Ferguson and C. A. Beveridge (2009)
Plant Physiology 149, 1929-1944
   Abstract »    Full Text »    PDF »
Rearrangement of Actin Cytoskeleton Mediates Invasion of Lotus japonicus Roots by Mesorhizobium loti.
K. Yokota, E. Fukai, L. H. Madsen, A. Jurkiewicz, P. Rueda, S. Radutoiu, M. Held, M. S. Hossain, K. Szczyglowski, G. Morieri, et al. (2009)
PLANT CELL 21, 267-284
   Abstract »    Full Text »    PDF »
Antiquity and Function of CASTOR and POLLUX, the Twin Ion Channel-Encoding Genes Key to the Evolution of Root Symbioses in Plants.
C. Chen, C. Fan, M. Gao, and H. Zhu (2009)
Plant Physiology 149, 306-317
   Abstract »    Full Text »    PDF »
From the Cover: CYCLOPS, a mediator of symbiotic intracellular accommodation.
K. Yano, S. Yoshida, J. Muller, S. Singh, M. Banba, K. Vickers, K. Markmann, C. White, B. Schuller, S. Sato, et al. (2008)
PNAS 105, 20540-20545
   Abstract »    Full Text »    PDF »
De Novo Organ Formation from Differentiated Cells: Root Nodule Organogenesis.
M. Crespi and F. Frugier (2008)
Science Signaling 1, re11
   Abstract »    Full Text »    PDF »
An autophagy-associated Atg8 protein is involved in the responses of Arabidopsis seedlings to hormonal controls and abiotic stresses.
S. Slavikova, S. Ufaz, T. Avin-Wittenberg, H. Levanony, and G. Galili (2008)
J. Exp. Bot.
   Abstract »    Full Text »    PDF »
Abscisic Acid Coordinates Nod Factor and Cytokinin Signaling during the Regulation of Nodulation in Medicago truncatula.
Y. Ding, P. Kalo, C. Yendrek, J. Sun, Y. Liang, J. F. Marsh, J. M. Harris, and G. E.D. Oldroyd (2008)
PLANT CELL 20, 2681-2695
   Abstract »    Full Text »    PDF »
EFD Is an ERF Transcription Factor Involved in the Control of Nodule Number and Differentiation in Medicago truncatula.
T. Vernie, S. Moreau, F. de Billy, J. Plet, J.-P. Combier, C. Rogers, G. Oldroyd, F. Frugier, A. Niebel, and P. Gamas (2008)
PLANT CELL 20, 2696-2713
   Abstract »    Full Text »    PDF »
Genome Structure of the Legume, Lotus japonicus.
S. Sato, Y. Nakamura, T. Kaneko, E. Asamizu, T. Kato, M. Nakao, S. Sasamoto, A. Watanabe, A. Ono, K. Kawashima, et al. (2008)
DNA Res 15, 227-239
   Abstract »    Full Text »    PDF »
3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase1 Interacts with NORK and Is Crucial for Nodulation in Medicago truncatula.
Z. Kevei, G. Lougnon, P. Mergaert, G. V. Horvath, A. Kereszt, D. Jayaraman, N. Zaman, F. Marcel, K. Regulski, G. B. Kiss, et al. (2007)
PLANT CELL 19, 3974-3989
   Abstract »    Full Text »    PDF »
Legumes Symbioses: Absence of Nod Genes in Photosynthetic Bradyrhizobia.
E. Giraud, L. Moulin, D. Vallenet, V. Barbe, E. Cytryn, J.-C. Avarre, M. Jaubert, D. Simon, F. Cartieaux, Y. Prin, et al. (2007)
Science 316, 1307-1312
   Abstract »    Full Text »    PDF »
Overlap of Proteome Changes in Medicago truncatula in Response to Auxin and Sinorhizobium meliloti.
G. E. van Noorden, T. Kerim, N. Goffard, R. Wiblin, F. I. Pellerone, B. G. Rolfe, and U. Mathesius (2007)
Plant Physiology 144, 1115-1131
   Abstract »    Full Text »    PDF »
Medicago truncatula NIN Is Essential for Rhizobial-Independent Nodule Organogenesis Induced by Autoactive Calcium/Calmodulin-Dependent Protein Kinase.
J. F. Marsh, A. Rakocevic, R. M. Mitra, L. Brocard, J. Sun, A. Eschstruth, S. R. Long, M. Schultze, P. Ratet, and G. E.D. Oldroyd (2007)
Plant Physiology 144, 324-335
   Abstract »    Full Text »    PDF »
A Cytokinin Perception Mutant Colonized by Rhizobium in the Absence of Nodule Organogenesis.
J. D. Murray, B. J. Karas, S. Sato, S. Tabata, L. Amyot, and K. Szczyglowski (2007)
Science 315, 101-104
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)