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Published Online September 21, 2006
Science DOI: 10.1126/science.1131794

Reports

Submitted on June 27, 2006
Accepted on September 8, 2006

Odorant Receptor-Derived cAMP Signals Direct Axonal Targeting

Takeshi Imai 1, Misao Suzuki 2, Hitoshi Sakano 1*

1 Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0032, Japan; CREST Program of Japan Science and Technology Agency, Tokyo 103-0027, Japan.
2 Division of Transgenic Technology, Center for Animal Resources and Development, Kumamoto University, Kumamoto 860-0811, Japan.

* To whom correspondence should be addressed.
Hitoshi Sakano , E-mail: sakano{at}mail.ecc.u-tokyo.ac.jp

In mammals, odorant receptors (ORs) direct axons of olfactory sensory neurons (OSNs) toward targets in the olfactory bulb. We show that G protein-mediated cAMP signals that regulate the expression of axon guidance molecules are essential for the OR-instructed axonal projection. Genetic manipulations of ORs, Gs, protein kinase A and a transcription factor, CREB, shifted the axonal projection sites along the anterior-posterior axis in the olfactory bulb. Thus it is the OR-derived cAMP signals, rather than direct action of OR molecules, that determines the target destinations of OSNs.


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