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Published Online September 7, 2006
Science DOI: 10.1126/science.1131127

Research Articles

Submitted on June 12, 2006
Accepted on August 9, 2006

Structure of the 70S Ribosome Complexed with mRNA and tRNA

Maria Selmer 1, Christine M. Dunham 1, Frank V. Murphy IV 1, Albert Weixlbaumer 1, Sabine Petry 1, Ann C. Kelley 1, John R. Weir 1, Venki Ramakrishnan 1*

1 MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom.

* To whom correspondence should be addressed.
Venki Ramakrishnan , E-mail: ramak{at}mrc-lmb.cam.ac.uk

The crystal structure of the bacterial 70S ribosome refined to 2.8 Å resolution reveals atomic details of its interactions with mRNA and tRNA. Metal ions stabilize the intersubunit interface as well as a kink in the mRNA that demarcates the boundary between the A and P sites, which is potentially important to prevent slippage of mRNA. The interactions of E-site tRNA with the 50S subunit have both similarities and differences compared to those in the archaeal ribosome. The structure also rationalizes much biochemical and genetic data on translation.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)