Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Published Online June 15, 2006
Science DOI: 10.1126/science.1126953

Reports

Submitted on March 2, 2006
Accepted on June 5, 2006

Structure of the Vesicular Stomatitis Virus Nucleoprotein-RNA Complex

Todd J. Green 1, Xin Zhang 1, Gail W. Wertz 2, Ming Luo 1*

1 Department of Microbiology, School of Medicine, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, AL 35294, USA.
2 Department of Microbiology, School of Medicine, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, AL 35294, USA; Present address: Dept. of Pathology, University of Virginia, MR5 Bldg. Rm 3051, Box 800904, 415 Lane Road, Charlottesville, VA 22908, USA.

* To whom correspondence should be addressed.
Ming Luo , E-mail: mingluo{at}uab.edu

Vesicular stomatitis virus is a negative-stranded RNA virus. Its nucleoprotein (N) binds the viral genomic RNA and is involved in multiple functions including transcription, replication, and assembly. We have determined a 2.9 Å structure of a complex containing 10 molecules of the N protein and 90 bases of RNA. The RNA is tightly sequestered in a cavity at the interface between two lobes of the N protein. This serves to protect the RNA in the absence of polynucleotide synthesis. In order for the RNA to be accessed, some conformational change in the N protein should be necessary.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Structure of the influenza virus A H5N1 nucleoprotein: implications for RNA binding, oligomerization, and vaccine design.
A. K.-L. Ng, H. Zhang, K. Tan, Z. Li, J.-h. Liu, P. K.-S. Chan, S.-M. Li, W.-Y. Chan, S. W.-N. Au, A. Joachimiak, et al. (2008)
FASEB J 22, 3638-3647
   Abstract »    Full Text »    PDF »
Cellular La Protein Shields Nonsegmented Negative-Strand RNA Viral Leader RNA from RIG-I and Enhances Virus Growth by Diverse Mechanisms.
V. Bitko, A. Musiyenko, M. A. Bayfield, R. J. Maraia, and S. Barik (2008)
J. Virol. 82, 7977-7987
   Abstract »    Full Text »    PDF »
Role of Intermolecular Interactions of Vesicular Stomatitis Virus Nucleoprotein in RNA Encapsidation.
X. Zhang, T. J. Green, J. Tsao, S. Qiu, and M. Luo (2008)
J. Virol. 82, 674-682
   Abstract »    Full Text »    PDF »
Cis-acting elements in the antigenomic promoter of Nipah virus.
P. Walpita and C. J. Peters (2007)
J. Gen. Virol. 88, 2542-2551
   Abstract »    Full Text »    PDF »
RSV604, a Novel Inhibitor of Respiratory Syncytial Virus Replication.
J. Chapman, E. Abbott, D. G. Alber, R. C. Baxter, S. K. Bithell, E. A. Henderson, M. C. Carter, P. Chambers, A. Chubb, G. S. Cockerill, et al. (2007)
Antimicrob. Agents Chemother. 51, 3346-3353
   Abstract »    Full Text »    PDF »
The 24-Angstrom Structure of Respiratory Syncytial Virus Nucleocapsid Protein-RNA Decameric Rings.
K. MacLellan, C. Loney, R. P. Yeo, and D. Bhella (2007)
J. Virol. 81, 9519-9524
   Abstract »    Full Text »    PDF »
Membrane and protein dynamics in live plant nuclei infected with Sonchus yellow net virus, a plant-adapted rhabdovirus.
M. M. Goodin, R. Chakrabarty, S. Yelton, K. Martin, A. Clark, and R. Brooks (2007)
J. Gen. Virol. 88, 1810-1820
   Abstract »    Full Text »    PDF »
Sequence-specific binding of single-stranded RNA: is there a code for recognition?.
S. D. Auweter, F. C. Oberstrass, and F. H.-T. Allain (2006)
Nucleic Acids Res. 34, 4943-4959
   Abstract »    Full Text »    PDF »



ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products


Science. ISSN 0036-8075 (print), 1095-9203 (online)