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Published Online June 15, 2006
Science DOI: 10.1126/science.1126953

Reports

Submitted on March 2, 2006
Accepted on June 5, 2006

Structure of the Vesicular Stomatitis Virus Nucleoprotein-RNA Complex

Todd J. Green 1, Xin Zhang 1, Gail W. Wertz 2, Ming Luo 1*

1 Department of Microbiology, School of Medicine, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, AL 35294, USA.
2 Department of Microbiology, School of Medicine, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, AL 35294, USA; Present address: Dept. of Pathology, University of Virginia, MR5 Bldg. Rm 3051, Box 800904, 415 Lane Road, Charlottesville, VA 22908, USA.

* To whom correspondence should be addressed.
Ming Luo , E-mail: mingluo{at}uab.edu

Vesicular stomatitis virus is a negative-stranded RNA virus. Its nucleoprotein (N) binds the viral genomic RNA and is involved in multiple functions including transcription, replication, and assembly. We have determined a 2.9 Å structure of a complex containing 10 molecules of the N protein and 90 bases of RNA. The RNA is tightly sequestered in a cavity at the interface between two lobes of the N protein. This serves to protect the RNA in the absence of polynucleotide synthesis. In order for the RNA to be accessed, some conformational change in the N protein should be necessary.


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