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Published Online June 8, 2006
Science DOI: 10.1126/science.1126747

Reports

Submitted on February 27, 2006
Accepted on May 26, 2006

Polo-Like Kinase Cdc5 Controls the Local Activation of Rho1 to Promote Cytokinesis

Satoshi Yoshida 1, Keiko Kono 2, Drew M. Lowery 3, Sara Bartolini 1, Michael B. Yaffe 3, Yoshikazu Ohya 4, David Pellman 1*

1 Department of Pediatric Oncology, Dana-Farber Cancer Institute and Division of Hematology/Oncology, Children's Hospital Boston and Harvard Medical School, Boston, MA 02115, USA.
2 Department of Pediatric Oncology, Dana-Farber Cancer Institute and Division of Hematology/Oncology, Children's Hospital Boston and Harvard Medical School, Boston, MA 02115, USA; Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277- 8562, Japan.
3 Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
4 Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277- 8562, Japan.

* To whom correspondence should be addressed.
David Pellman , E-mail: david_pellman{at}dfci.harvard.edu

The links between the cell cycle machinery and the cytoskeletal proteins controlling cytokinesis are poorly understood. The small G-protein RhoA stimulates type II myosin contractility and formin-dependent assembly of the cytokinetic actin contractile ring. Here, we found that budding yeast Polo-like kinase Cdc5 controls the targeting and activation of Rho1 (RhoA) at the division site via Rho1 guanine nucleotide exchange factors. This role of Cdc5 (Polo) in regulating Rho1 is likely to be relevant to cytokinesis and asymmetric cell division in other organisms.


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