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Submitted on November 21, 2005
Accepted on April 21, 2006
AXR4 Is Required for Localization of the Auxin Influx Facilitator AUX1
S. Dharmasiri 1, R. Swarup 2, K. Mockaitis 3, N. Dharmasiri 1, S. K. Singh 4, M. Kowalchyk 4, A. Marchant 4, S. Mills 5, G. Sandberg 6, M. J. Bennett 2*, M. Estelle 3*
1 Department of Biology, Indiana University, USA; Present address: Department of Biology, Texas State University-San Marcos, USA. 2 School of Biosciences, University of Nottingham, UK. 3 Department of Biology, Indiana University, USA. 4 Umeå Plant Science Centre, SLU, Umeå, Sweden. 5 School of Computer Science and Information Technology, University of Nottingham, UK. 6 Umeå Plant Science Centre, SLU, Umeå, Sweden.
* To whom correspondence should be addressed.
M. J. Bennett , E-mail: malcolm.bennett{at}nottingham.ac.uk M. Estelle , E-mail: maestell{at}indiana.edu
The AUX1 and PIN auxin influx and efflux facilitators are keyregulators of root growth and development. Root gravitropismrequires AUX1 and PIN2 to transport auxin via the lateral rootcap to elongating epidermal cells. Genetic studies suggest thatAXR4 functions in the same pathway as AUX1. Here we show thatAXR4 is a novel ER accessory protein that regulates localizationof AUX1, but not PIN proteins. Loss of AXR4 results in abnormalaccumulation of AUX1 in the ER of epidermal cells indicatingthat the axr4 agravitropic phenotype is due to a defect in AUX1trafficking in the root epidermis.
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