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Published Online March 23, 2006
Science DOI: 10.1126/science.1122352

Reports

Submitted on November 8, 2005
Accepted on March 2, 2006

A Voltage Sensor-Domain Protein is a Voltage-Gated Proton Channel

Mari Sasaki 1, Masahiro Takagi 2, Yasushi Okamura 1*

1 Section of Developmental Neurophysiology, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Higashiyama 5-1, Myodaiji-cho, Okazaki, Aichi 444-8787, Japan; The Graduate University for Advanced Studies, Higashiyama 5-1, Myodaiji-cho, Okazaki 444-8787, Japan; National Institute for Physiological Sciences, National Institutes of Natural Sciences, Higashiyama 5-1, Myodaiji-cho, Okazaki, Aichi 444-8787, Japan.
2 Section of Developmental Neurophysiology, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Higashiyama 5-1, Myodaiji-cho, Okazaki, Aichi 444-8787, Japan; National Institute for Physiological Sciences, National Institutes of Natural Sciences, Higashiyama 5-1, Myodaiji-cho, Okazaki, Aichi 444-8787, Japan.

* To whom correspondence should be addressed.
Yasushi Okamura , E-mail: yokamura{at}nips.ac.jp

Voltage-gated proton channels have been widely observed, but have not been identified at a molecular level. Here we report that a four transmembrane protein similar to the voltage sensor domain of voltage-gated ion channels is a voltage-gated proton channel. Cells overexpressing this protein showed depolarization-induced outward currents accompanied by tail currents. Current reversal occurs at equilibrium potentials for protons. The currents exhibit pH-dependent gating and Zn2+ sensitivity, two features characteristic of voltage-gated proton channels. Responses of voltage dependence to sequence changes suggest that mVSOP is itself a channel, rather than a regulator of another channel protein.


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