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Published Online October 27, 2005
Science DOI: 10.1126/science.1120499

Research Articles

Submitted on September 23, 2005
Accepted on October 19, 2005

Logic of the Yeast Metabolic Cycle: Temporal Compartmentalization of Cellular Processes

Benjamin P. Tu 1, Andrzej Kudlicki 1, Maga Rowicka 1, Steven L. McKnight 1*

1 Department of Biochemistry, UT Southwestern Medical Center, 5323 Harry Hines Blvd., L3.124, Dallas, TX 75390 USA.

* To whom correspondence should be addressed.
Steven L. McKnight , E-mail: smckni{at}biochem.swmed.edu

Budding yeast grown under continuous, nutrient-limited conditions exhibit robust, highly periodic cycles in the form of respiratory bursts. Microarray studies reveal that over half of the yeast genome is expressed periodically during these metabolic cycles. Genes encoding proteins having a common function exhibit similar temporal expression patterns, and genes specifying functions associated with energy and metabolism tend to be expressed with exceptionally robust periodicity. Essential cellular and metabolic events occur in synchrony with the metabolic cycle, demonstrating that key processes in a simple eukaryotic cell are compartmentalized in time. Thus, we describe an ultradian metabolic cycle that is fundamentally important to the life of a cell.


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