Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Published Online October 27, 2005
Science DOI: 10.1126/science.1119969

Reports

Submitted on September 9, 2005
Accepted on October 20, 2005

Golgi Duplication in Trypanosoma brucei Requires Centrin2

Cynthia Y. He 1, Marc Pypaert 1, Graham Warren 1*

1 Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA.

* To whom correspondence should be addressed.
Graham Warren , E-mail: graham.warren{at}yale.edu

Centrins are highly conserved components of the centrosome, which, in the parasitic protozoan T. brucei, comprises the basal body and nucleates the flagellum used for locomotion. Here we found TbCentrin2 in an additional bi-lobed structure near to the Golgi apparatus. One lobe was associated with the old Golgi, the other became associated with the newly forming Golgi, as the cell grew. Depletion of TbCentrin1 inhibited duplication of the basal body whereas depletion of TbCentrin2 also inhibited duplication of the Golgi. Thus a Centrin2-containing structure distinct from the basal body appears to mark the site for new Golgi assembly.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Centrin4 coordinates cell and nuclear division in T. brucei.
J. Shi, J. B. Franklin, J. T. Yelinek, I. Ebersberger, G. Warren, and C. Y. He (2008)
J. Cell Sci. 121, 3062-3070
   Abstract »    Full Text »    PDF »
Polo-like kinase is required for Golgi and bilobe biogenesis in Trypanosoma brucei.
C. L. de Graffenried, H. H. Ho, and G. Warren (2008)
J. Cell Biol. 181, 431-438
   Abstract »    Full Text »    PDF »
Intraflagellar Transport and Functional Analysis of Genes Required for Flagellum Formation in Trypanosomes.
S. Absalon, T. Blisnick, L. Kohl, G. Toutirais, G. Dore, D. Julkowska, A. Tavenet, and P. Bastin (2008)
Mol. Biol. Cell 19, 929-944
   Abstract »    Full Text »    PDF »
Functional diversification of centrins and cell morphological complexity.
D. Gogendeau, C. Klotz, O. Arnaiz, A. Malinowska, M. Dadlez, N. G. de Loubresse, F. Ruiz, F. Koll, and J. Beisson (2008)
J. Cell Sci. 121, 65-74
   Abstract »    Full Text »    PDF »
Tousled-like kinase in a microbial eukaryote regulates spindle assembly and S-phase progression by interacting with Aurora kinase and chromatin assembly factors.
Z. Li, S. Gourguechon, and C. C. Wang (2007)
J. Cell Sci. 120, 3883-3894
   Abstract »    Full Text »    PDF »
Stability and Robustness of an Organelle Number Control System: Modeling and Measuring Homeostatic Regulation of Centriole Abundance.
W. F. Marshall (2007)
Biophys. J. 93, 1818-1833
   Abstract »    Full Text »    PDF »
Centrin1 Is Required for Organelle Segregation and Cytokinesis in Trypanosoma brucei.
A. Selvapandiyan, P. Kumar, J. C. Morris, J. L. Salisbury, C. C. Wang, and H. L. Nakhasi (2007)
Mol. Biol. Cell 18, 3290-3301
   Abstract »    Full Text »    PDF »
Trypanosoma brucei ARF1 Plays a Central Role in Endocytosis and Golgi-Lysosome Trafficking.
H. P. Price, M. Stark, and D. F. Smith (2007)
Mol. Biol. Cell 18, 864-873
   Abstract »    Full Text »    PDF »
Ordered assembly of the duplicating Golgi in Trypanosoma brucei.
H. H. Ho, C. Y. He, C. L. de Graffenried, L. J. Murrells, and G. Warren (2006)
PNAS 103, 7676-7681
   Abstract »    Full Text »    PDF »
Golgi relies on centrin.
N. LeBrasseur (2005)
J. Cell Biol. 171, 583
   Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)