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Published Online October 27, 2005
Science DOI: 10.1126/science.1119969

Reports

Submitted on September 9, 2005
Accepted on October 20, 2005

Golgi Duplication in Trypanosoma brucei Requires Centrin2

Cynthia Y. He 1, Marc Pypaert 1, Graham Warren 1*

1 Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA.

* To whom correspondence should be addressed.
Graham Warren , E-mail: graham.warren{at}yale.edu

Centrins are highly conserved components of the centrosome, which, in the parasitic protozoan T. brucei, comprises the basal body and nucleates the flagellum used for locomotion. Here we found TbCentrin2 in an additional bi-lobed structure near to the Golgi apparatus. One lobe was associated with the old Golgi, the other became associated with the newly forming Golgi, as the cell grew. Depletion of TbCentrin1 inhibited duplication of the basal body whereas depletion of TbCentrin2 also inhibited duplication of the Golgi. Thus a Centrin2-containing structure distinct from the basal body appears to mark the site for new Golgi assembly.


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