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Published Online November 17, 2005
Science DOI: 10.1126/science.1118313

Reports

Submitted on August 2, 2005
Accepted on November 4, 2005

Glial Membranes at the Node of Ranvier Prevent Neurite Outgrowth

Jeffrey K. Huang 1, Greg R. Phillips 2, Alejandro D. Roth 3, Liliana Pedraza 3, Weisong Shan 3, Wiam Belkaid 3, Sha Mi 4, Asa Fex-Svenningsen 5, Laurence Florens 6, John R. Yates III 7, David R. Colman 8*

1 Fishberg Department of Neuroscience, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA; Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK.
2 Fishberg Department of Neuroscience, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA.
3 The Montreal Neurological Institute, McGill University, 3801 University Street, Montreal PQ H3A 2B4, Canada.
4 Biogen Idec, Discovery Biology, 14 Cambridge Center, Cambridge, MA 02142, USA.
5 Department of Genetics and Pathology, Rudbeck laboratory, Uppsala University, SE-751 85 Uppsala, Sweden.
6 Stowers Institute, 1000 East 50th Street, Kansas City, MO 64110, USA.
7 Department of Cell Biology SR11, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
8 Fishberg Department of Neuroscience, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA; The Montreal Neurological Institute, McGill University, 3801 University Street, Montreal PQ H3A 2B4, Canada.

* To whom correspondence should be addressed.
David R. Colman , E-mail: david.colman{at}mcgill.ca

Nodes of Ranvier are regularly placed, nonmyelinated axon segments along myelinated nerves. Here we show that nodal membranes isolated from the central nervous system (CNS) of mammals restricted neurite outgrowth of cultured neurons. Proteomic analysis of these membranes revealed several inhibitors of neurite outgrowth, including the oligodendrocyte myelin glycoprotein (OMgp). In brain, OMgp was not localized to compact myelin as previously thought, but to oligodendroglia-like cells, whose processes converge to form a ring that completely encircles the nodes. In OMgp null mice, CNS nodes were abnormally wide and collateral sprouting was observed. Nodal ensheathment in the CNS may stabilize the node and prevent axonal sprouting.


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