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Published Online July 14, 2005
Science DOI: 10.1126/science.1116006

Reports

Submitted on June 13, 2005
Accepted on July 7, 2005

Drosophila RNAi Screen Reveals CD36 Family Member Required for Mycobacterial Infection

Jennifer A. Philips 1*, Eric J. Rubin 2, Norbert Perrimon 3*

1 Department of Genetics
2 Department of Immunology and Infectious Disease, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA.
3 Department of Genetics; Howard Hughes Medical Institute, Harvard Medical School, 77 Louis Pasteur Avenue, Boston, MA 02115, USA.

* To whom correspondence should be addressed.
Jennifer A. Philips , E-mail: jphilips{at}partners.org
Norbert Perrimon , E-mail: perrimon{at}receptor.med.harvard.edu

Certain pathogens, such as Mycobacterium tuberculosis, survive within the hostile intracellular environment of a macrophage. To identify host factors required for mycobacterial entry and survival within macrophages, we performed a genomewide RNAi screen in Drosophila macrophage-like cells, using Mycobacterium fortuitum. We identified factors required for general phagocytosis, as well as those needed specifically for mycobacterial infection. One specific factor, Peste (Pes), is a CD36 family member required for uptake of mycobacteria, but not E. coli or S. aureus. Moreover, mammalian class B scavenger receptors (SRs) conferred uptake of bacteria to nonphagocytic cells, with SR-BI and SR-BII uniquely mediating uptake of M. fortuitum, which suggests a conserved role for class B SRs in pattern recognition and innate immunity.


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