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Published Online September 1, 2005
Science DOI: 10.1126/science.1114758

Reports

Submitted on May 12, 2005
Accepted on August 9, 2005

Direct Isolation of Satellite Cells For Skeletal Muscle Regeneration

Didier Montarras 1*, Jennifer Morgan 2, Charlotte Collins 3, Frédéric Relaix 1, Stéphane Zaffran 1, Ana Cumano 4, Terence Partridge 3, Margaret Buckingham 1*

1 CNRS URA 2578, Department of Developmental Biology, Pasteur Institute, 75724 Paris Cedex 15, France.
2 Department of Paediatrics, Imperial College London, The Dubowitz Neuromuscular Centre, Hammersmith Hospital, Du Cane Road, London W12 ONN, UK; Muscle Cell Biology Group, MRC Clinical Sciences Centre, Imperial College, Du Cane Road, London W12 ONN, UK.
3 Muscle Cell Biology Group, MRC Clinical Sciences Centre, Imperial College, Du Cane Road, London W12 ONN, UK.
4 Unité du Développement des Lymphocytes, U 668 INSERM Pasteur Institute, 75724 Paris Cedex 15, France.

* To whom correspondence should be addressed.
Didier Montarras , E-mail: dmontarr{at}pasteur.fr
Margaret Buckingham , E-mail: margab{at}pasteur.fr

Muscle satellite cells contribute to muscle regeneration. We have used a Pax3GFP/+ mouse line to directly isolate (Pax3)GFP expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as a homogeneous population of small, non-granular, Pax7+, CD34+, CD45-, Sca1- cells. The flow cytometry parameters thus established, enabled us to isolate satellite cells from wild type muscles. Such cells, grafted into muscles of mdx nu/nu mice contribute both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture prior to engraftment reduces their regenerative capacity.


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