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Published Online May 19, 2005
Science DOI: 10.1126/science.1113346

Reports

Submitted on April 8, 2005
Accepted on May 9, 2005

tRNA Actively Shuttles Between the Nucleus and Cytosol in Yeast

Akira Takano 1, Toshiya Endo 2, Tohru Yoshihisa 3*

1 Department of Chemistry, Graduate School of Science, Nagoya University, Nagoya, 464-8602, Japan.
2 Department of Chemistry, Graduate School of Science, Nagoya University, Nagoya, 464-8602, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Japan; Institute for Advanced Research, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
3 Department of Chemistry, Graduate School of Science, Nagoya University, Nagoya, 464-8602, Japan; Research Center for Materials Science, Nagoya University, Nagoya, 464-8602, Japan.

* To whom correspondence should be addressed.
Tohru Yoshihisa , E-mail: tyoshihi{at}biochem.chem.nagoya-u.ac.jp

Previous evidence suggested that transfer RNAs (tRNAs) cross the nuclear envelope to the cytosol only once after maturing in the nucleus. We now present evidence for nuclear import of tRNAs in yeast. Several export mutants accumulate mature tRNAs in the nucleus even in the absence of transcription. Import requires energy but not the Ran cycle. These results indicate that tRNAs shuttle between the nucleus and cytosol.


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