Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Published Online May 19, 2005
Science DOI: 10.1126/science.1112286

Reports

Submitted on March 15, 2005
Accepted on May 12, 2005

Patient-Specific Embryonic Stem Cells Derived from Human SCNT Blastocysts

Woo Suk Hwang 1*, Sung Il Roh 2, Byeong Chun Lee 3, Sung Keun Kang 3, Dae Kee Kwon 3, Sue Kim 3, Sun Jong Kim 2, Sun Woo Park 3, Hee Sun Kwon 3, Chang Kyu Lee 4, Jung Bok Lee 2, Jin Mee Kim 2, Curie Ahn 5, Sun Ha Paek 5, Sang Sik Chang 6, Jung Jin Koo 6, Hyun Soo Yoon 7, Jung Hye Hwang 7, Youn Young Hwang 7, Ye Soo Park 7, Sun Kyung Oh 5, Hee Sun Kim 5, Jong Hyuk Park 8, Shin Yong Moon 5, Gerald Schatten 8*

1 College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea; School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
2 Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea.
3 College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
4 School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
5 College of Medicine, Seoul National University, Seoul, 110-744, Korea.
6 Hanna Women's Clinic, Seoul, 137-872, Korea.
7 School of Medicine, Hanyang University, Seoul, 471-701, Korea.
8 Pittsburgh Development Center, Magee-Womens Research Institute, Departments of Obstetrics-Gynecology-Reproductive Sciences and Cell Biology-Physiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213 USA.

* To whom correspondence should be addressed.
Woo Suk Hwang , E-mail: hwangws{at}snu.ac.kr
Gerald Schatten , E-mail: gschatten{at}pdc.magee.edu

Patient-specific, immune-matched human embryonic stem cells (hESC) are anticipated to be of great biomedical importance for studies of disease and development, and to advance clinical deliberations for stem cell transplantation. Eleven hESC lines were established by nuclear transfer (SCNT; NT) of skin cells from patients with disease or injury into donated oocytes. These lines (NT-hESCs), grown on human feeders from the same NT-donor or genetically-unrelated individuals, were established at high rates, regardless of NT-donor sex or age. NT-hESCs were pluripotent, chromosomally normal, and match NT-patient's DNA. Major Histocompatibility Complex (MHC) identity of each NT-hESC with the patient's showed immunological compatibility, important for eventual transplantation. With the generation of these NT-hESCs, evaluations of genetic and epigenetic stability can be made. Additional work remains regarding the development of reliable directed differentiation and the elimination of remaining animal components. Prior to clinical use of these cells, preclinical evidence is required to prove that transplantation of differentiated-NT-hESCs can be safe, effective and tolerated.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Public feeling for science: The Hwang affair and Hwang supporters.
J. Kim (2009)
Public Understanding of Science 18, 670-686
   Abstract »    PDF »
Sex and reproduction: an evolving relationship.
G. Benagiano, S. Carrara, and V. Filippi (2009)
Hum. Reprod. Update
   Abstract »    Full Text »    PDF »
What happened to the stem cells?.
T Hviid Nielsen (2008)
J. Med. Ethics 34, 852-857
   Abstract »    Full Text »    PDF »
Authorship: why not just toss a coin?.
K. Strange (2008)
Am J Physiol Cell Physiol 295, C567-C575
   Full Text »    PDF »
Pluripotency and nuclear reprogramming.
S. Yamanaka (2008)
Phil Trans R Soc B 363, 2079-2087
   Abstract »    Full Text »    PDF »
The road to pluripotence: the research response to the embryonic stem cell debate.
C. T. Scott and R. A. Reijo Pera (2008)
Hum. Mol. Genet. 17, R3-R9
   Abstract »    Full Text »    PDF »
Research ethics and lessons from Hwanggate: what can we learn from the Korean cloning fraud?.
R Saunders and J Savulescu (2008)
J. Med. Ethics 34, 214-221
   Abstract »    Full Text »    PDF »
From Revered to Reviled: A Cross-Cultural Narrative Analysis of the South Korean Cloning Scandal.
M. Kruvand and S. Hwang (2007)
Science Communication 29, 177-197
   Abstract »    PDF »
Hwang case review committee misses the mark.
M. Rossner (2007)
J. Cell Biol. 176, 131-132
   Full Text »    PDF »
Correction of sickle cell disease by homologous recombination in embryonic stem cells.
L.-C. Wu, C.-W. Sun, T. M. Ryan, K. M. Pawlik, J. Ren, and T. M. Townes (2006)
Blood 108, 1183-1188
   Abstract »    Full Text »    PDF »
Role of Histone Acetylation in Reprogramming of Somatic Nuclei Following Nuclear Transfer.
A. Rybouchkin, Y. Kato, and Y. Tsunoda (2006)
Biol Reprod 74, 1083-1089
   Abstract »    Full Text »    PDF »
Stem Cell and Related Therapies: Nurses and midwives representing all parties.
S H Cedar (2006)
Nursing Ethics 13, 292-303
   Abstract »    PDF »
Why the apparent haste to clone humans?.
N Cobbe (2006)
J. Med. Ethics 32, 298-302
   Full Text »    PDF »
Science fraud: from patchwork mouse to patchwork data.
G. Weissmann (2006)
FASEB J 20, 587-590
   Full Text »    PDF »
Mirror exchange of donor gametes should also accommodate scientific research..
B. C. Heng and G. Q. Tong (2006)
Hum. Reprod. 21, 1100
   Full Text »    PDF »
Embryonic stem-cell gametes: the new frontier in human reproduction.
Z. Master (2006)
Hum. Reprod. 21, 857-863
   Abstract »    Full Text »    PDF »
Scientific and clinical opportunities for modeling blood disorders with embryonic stem cells.
M. W. Lensch and G. Q. Daley (2006)
Blood 107, 2605-2612
   Abstract »    Full Text »    PDF »
Directed Differentiation of Human Embryonic Stem Cells into Functional Dendritic Cells through the Myeloid Pathway..
I. I. Slukvin, M. A. Vodyanik, J. A. Thomson, M. E. Gumenyuk, and K.-D. Choi (2006)
J. Immunol. 176, 2924-2932
   Abstract »    Full Text »    PDF »
Designer blood: creating hematopoietic lineages from embryonic stem cells.
A. L. Olsen, D. L. Stachura, and M. J. Weiss (2006)
Blood 107, 1265-1275
   Abstract »    Full Text »    PDF »
Are journals doing enough to prevent fraudulent publication?.
(2006)
Can. Med. Assoc. J. 174, 431
   Full Text »    PDF »
Les journaux font-ils assez d'efforts pour prevenir la publication frauduleuse?.
(2006)
Can. Med. Assoc. J. 174, 433
   Full Text »    PDF »
Derivation of human embryonic stem cell lines from embryos obtained after IVF and after PGD for monogenic disorders.
I. Mateizel, N. De Temmerman, U. Ullmann, G. Cauffman, K. Sermon, H. Van de Velde, M. De Rycke, E. Degreef, P. Devroey, I. Liebaers, et al. (2006)
Hum. Reprod. 21, 503-511
   Abstract »    Full Text »    PDF »
Beyond Fraud -- Stem-Cell Research Continues.
E. Y. Snyder and J. F. Loring (2006)
N. Engl. J. Med. 354, 321-324
   Full Text »    PDF »
Egg Donation and Human Embryonic Stem-Cell Research.
R. Steinbrook (2006)
N. Engl. J. Med. 354, 324-326
   Full Text »    PDF »
Korean cloning studies were fakes.
J. Parry (2006)
BMJ 332, 67
   Full Text »    PDF »
Korean stem cell pioneer resigns.
J. Parry (2006)
BMJ 332, 7
   Full Text »    PDF »
Temporal and parental-specific expression of imprinted genes in a newly derived Chinese human embryonic stem cell line and embryoid bodies.
B. W. Sun, A. C. Yang, Y. Feng, Y. J. Sun, Y. f. Zhu, Y. Zhang, H. Jiang, C. L. Li, F. R. Gao, Z. H. Zhang, et al. (2006)
Hum. Mol. Genet. 15, 65-75
   Abstract »    Full Text »    PDF »
Engineering Myocardial Tissue.
T. Eschenhagen and W. H. Zimmermann (2005)
Circ. Res. 97, 1220-1231
   Abstract »    Full Text »    PDF »
Global gene expression profiles reveal significant nuclear reprogramming by the blastocyst stage after cloning.
S. L. Smith, R. E. Everts, X. C. Tian, F. Du, L.-Y. Sung, S. L. Rodriguez-Zas, B.-S. Jeong, J.-P. Renard, H. A. Lewin, and X. Yang (2005)
PNAS 102, 17582-17587
   Abstract »    Full Text »    PDF »
Stem Cell Research: Paths to Cancer Therapies and Regenerative Medicine.
I. Weissman (2005)
JAMA 294, 1359-1366
   Abstract »    Full Text »    PDF »
The CMA Code of Ethics and the donation of fresh embryos for stem cell research.
J. Nisker and A. White (2005)
Can. Med. Assoc. J. 173, 621-622
   Full Text »    PDF »
Nuclear Reprogramming of Somatic Cells After Fusion with Human Embryonic Stem Cells.
C. A. Cowan, J. Atienza, D. A. Melton, and K. Eggan (2005)
Science 309, 1369-1373
   Abstract »    Full Text »    PDF »
In brief.
(2005)
BMJ 330, 1346
   Full Text »    PDF »
UK and Korean teams refine techniques for human cloning.
S. Mayor (2005)
BMJ 330, 1225
   Full Text »    PDF »

E-Letters:

Read all E-Letters

Egg-sharing to alleviate the shortage of donor oocytes for therapeutic cloning
Boon C. Heng, et al.
Science Online, 24 May 2005 [Full text]

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)