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Published Online April 7, 2005
Science DOI: 10.1126/science.1108408

Reports

Submitted on December 7, 2004
Accepted on March 30, 2005

Kinesin and Dynein Move a Peroxisome in Vivo: A Tug-of-War or Coordinated Movement?

Comert Kural 1, Hwajin Kim 2, Sheyum Syed 3, Gohta Goshima 4, Vladimir I. Gelfand 5*, Paul R. Selvin 6*

1 Biophysics Center
2 Department of Cell and Structural Biology, University of Illinois, Urbana, IL 61801, USA.
3 Physics Department
4 Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94107, USA.
5 Department of Cell and Structural Biology, University of Illinois, Urbana, IL 61801, USA; Present address: Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
6 Biophysics Center; Physics Department

* To whom correspondence should be addressed.
Vladimir I. Gelfand , E-mail: vgelfand{at}northwestern.edu
Paul R. Selvin , E-mail: selvin{at}uiuc.edu

We have used Fluorescence Imaging with One Nanometer Accuracy (FIONA) for analysis of organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We can locate a green fluorescence protein (GFP)-tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to determine the average step size to be ~8 nanometers for both dynein and kinesin. Furthermore, we find that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, we find that multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed.


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