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Published Online January 27, 2005
Science DOI: 10.1126/science.1107580

Reports

Submitted on November 16, 2004
Accepted on January 18, 2005

BZR1 is a Transcriptional Repressor with Dual Roles in Brassinosteroid Homeostasis and Growth Response

Jun-Xian He 1, Joshua M. Gendron 2, Yu Sun 1, Srinivas S. L. Gampala 1, Nathan Gendron 1, Catherine Qing Sun 1, Zhi-Yong Wang 1*

1 Department of Plant Biology, Carnegie Institution, Stanford, CA 94305, USA.
2 Department of Plant Biology, Carnegie Institution, Stanford, CA 94305, USA; Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA.

* To whom correspondence should be addressed.
Zhi-Yong Wang , E-mail: zywang24{at}stanford.edu

Brassinosteroid (BR) homeostasis and signaling are crucial for normal growth and development of plants. BR signaling through cell-surface receptor kinases and intracellular components leads to dephosphorylation and accumulation of the nuclear protein BZR1. How BR signaling regulates gene expression, however, remains unknown. Here we show that BZR1 is a transcriptional repressor that has a previously unknown DNA binding domain and binds directly to the promoters of feedback-regulated BR biosynthetic genes. Microarray analyses identified additional potential targets of BZR1 and illustrated, together with physiological studies, that BZR1 coordinates BR homeostasis and signaling by playing dual roles in regulating BR biosynthesis and downstream growth responses.


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