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Published Online March 11, 2004
Science DOI: 10.1126/science.1095801

Reports

Submitted on January 20, 2004
Accepted on March 1, 2004

Transmembrane Segments of Syntaxin Line the Fusion Pore of Ca2+-Triggered Exocytosis

Xue Han 1, Chih-Tien Wang 1, Jihong Bai 1, Edwin R. Chapman 1, Meyer B. Jackson 1*

1 Department of Physiology, University of Wisconsin, 1300 University Avenue, Madison, WI 53706, USA.

* To whom correspondence should be addressed. E-mail: mjackson{at}physiology.wisc.edu.

The fusion pore of regulated exocytosis is a channel that connects and spans the vesicle and plasma membranes. The molecular composition of this important intermediate structure of exocytosis is unknown. Here we found that mutations of some residues within the transmembrane segment of syntaxin (Syx), a plasma membrane protein essential for exocytosis, altered neurotransmitter flux through fusion pores and altered pore conductance. The residues that influenced fusion pore flux lay along one face of an {alpha}-helical model. Thus the fusion pore is formed at least in part by a circular arrangement of 5-8 Syx transmembrane segments in the plasma membrane.


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