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Published Online April 15, 2004
Science DOI: 10.1126/science.1095191

Research Articles

Submitted on December 30, 2003
Accepted on March 26, 2004

Oscillating Global Regulators Control the Genetic Circuit Driving a Bacterial Cell Cycle

Julia Holtzendorff 1, Dean Hung 1, Peter Brende 1, Ann Reisenauer 1, Patrick H. Viollier 1, Harley H. McAdams 1, Lucy Shapiro 1*

1 Department of Developmental Biology, Stanford University School of Medicine, Beckman Center, 279 Campus Drive, Stanford, CA 94305, USA.

* To whom correspondence should be addressed.
Lucy Shapiro , E-mail: shapiro{at}stanford.edu

A newly identified cell cycle master regulator protein, GcrA, together with the CtrA master regulator, are key components of a genetic circuit that drives cell cycle progression and asymmetric polar morphogenesis in Caulobacter crescentus. The circuit drives out of phase temporal and spatial oscillation of GcrA and CtrA concentrations producing time and space dependent transcriptional regulation of modular functions that implement cell cycle processes. The CtrA/GcrA regulatory circuit controls expression of polar differentiation factors and the timing of DNA replication. CtrA functions as a silencer of the replication origin and GcrA as an activator of components of the replisome and the segregation machinery.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)