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Published Online February 27, 2003
Science DOI: 10.1126/science.1080418

Reports

Submitted on November 13, 2002
Accepted on February 13, 2003

Asymmetric Inheritance of Oxidatively Damaged Proteins During Cytokinesis

Hugo Aguilaniu 1, Lena Gustafsson 2, Michel Rigoulet 3, Thomas Nyström 4*

1 Department of Cell and Molecular Biology-Microbiology, Göteborg University, Göteborg, Sweden; Department of Molecular Biotechnology, Chalmers University of Technology, Göteborg, Sweden; Institut de Biochimie et de Génétiques Cellulaires du CNRS, Université Victor Segalen, Bordeaux, France.
2 Department of Molecular Biotechnology, Chalmers University of Technology, Göteborg, Sweden.
3 Institut de Biochimie et de Génétiques Cellulaires du CNRS, Université Victor Segalen, Bordeaux, France.
4 Department of Cell and Molecular Biology-Microbiology, Göteborg University, Göteborg, Sweden.

* To whom correspondence should be addressed. E-mail: thomas.nystrom{at}gmm.gu.se.

Carbonylated proteins were visualized in single cells of the budding yeast Saccharomyces cerevisiae, revealing that they accumulate with replicative age. Furthermore, carbonylated proteins were not inherited by daughter cells during cytokinesis. Mother cells of a yeast strain lacking the sir2 gene, a life-span determinant, failed to retain oxidatively damaged proteins during cytokinesis. These findings suggest that a genetically determined, Sir2p-dependent asymmetric inheritance of oxidatively damaged proteins may contribute to free-radical defense and the fitness of newborn cells.


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