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Published Online November 14, 2002
Science DOI: 10.1126/science.1077642

Reports

Submitted on August 22, 2002
Accepted on November 1, 2002

Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation

Paul Wentworth Jr. 1, Jonathan E. McDunn 1, Anita D. Wentworth 1, Cindy Takeuchi 2, Jorge Nieva 3, Teresa Jones 1, Cristina Bautista 1, Julie M. Ruedi 3, Abel Gutierrez 3, Kim D. Janda 1, Bernard M. Babior 3, Albert Eschenmoser 4, Richard A. Lerner 1

1 Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
2 Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
3 Department of Molecular and Experimental Medicine and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
4 Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA; Laboratorium fuer organische Chemie, ETH Zuerich, Universitaetstr. 16 CH-8092 Zuerich, Switzerland.

Recently we discovered that antibodies catalyze the generation of hydrogen peroxide (H2O2) from singlet molecular oxygen (1O2*) and water. Here we show this process can lead to efficient killing of bacteria, regardless of the antigen specificity of the antibody. H2O2 production by antibodies was not alone sufficient for bacterial killing and further studies suggested that the antibody-catalyzed water-oxidation pathway produced an additional molecular species with a chemical signature similar to that of ozone. This species is also generated during the oxidative burst of activated human neutrophils and during inflammation. These observations suggest that alternative pathways may exist for biological killing of bacteria that are mediated by potent oxidants new to biology.


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