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Published Online August 8, 2002
Science DOI: 10.1126/science.1075119

Reports

Submitted on June 17, 2002
Accepted on July 29, 2002

Role of ANC-1 in Tethering Nuclei to the Actin Cytoskeleton

Daniel A. Starr 1 Min Han 1*

1 Howard Hughes Medical Institute and Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA.

* To whom correspondence should be addressed. E-mail: mhan{at}colorado.edu.

Mutations in anc-1 (nuclear anchorage defective) disrupt the positioning of nuclei and mitochondria in Caenorhabditis elegans. Here anc-1 was shown to consist of mostly coiled regions with a nuclear envelope localization domain (KASH domain) and an actin-binding domain; this structure was conserved with Drosophila Msp-300 and mammalian Syne-1. Antibodies against ANC-1 localized cytoplasmically, and were enriched at the nuclear periphery in an UNC-84 dependent manner. Overexpression of the KASH domain or the actin-binding domain caused a dominant negative anchorage defect. Thus ANC-1 may connect nuclei to the cytoskeleton by interacting with UNC-84 at the nuclear envelope and actin in the cytoplasm.


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