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Published Online August 22, 2002
Science DOI: 10.1126/science.1074973

Research Articles

Submitted on June 12, 2002
Accepted on August 9, 2002

Regulation of Heterochromatic Silencing and Histone H3 Lysine-9 Methylation by RNAi

Tom Volpe 1, Catherine Kidner 1, Ira M. Hall 2, Grace Teng 2, Shiv I. S. Grewal 1*, Rob Martienssen 1*

1 Cold Spring Harbor Laboratory, Cold Spring Harbor NY 11724, USA.
2 Cold Spring Harbor Laboratory, Watson School of Biological Sciences, Cold Spring Harbor NY 11724, USA.

* To whom correspondence should be addressed. E-mail: grewal{at}cshl.org, martiens{at}cshl.org.

Eukaryotic heterochromatin is characterized by a high density of repeats and transposons, as well as by modified histones, and influences both gene expression and chromosome segregation. We have deleted the argonaute, dicer and RNA-dependent RNA polymerase gene homologs in the fission yeast Schizosaccharomyces pombe, which encode part of the machinery responsible for RNA interference (RNAi). Deletion results in the aberrant accumulation of complementary transcripts from centromeric heterochromatic repeats. This is accompanied by transcriptional de-repression of transgenes integrated at the centromere, loss of histone H3 lysine 9 methylation, and impairment of centromere function. We propose that double stranded RNA (dsRNA) arising from centromeric repeats targets formation and maintenance of heterochromatin through RNAi.


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