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Published Online January 3, 2002
Science DOI: 10.1126/science.1068228

Reports

Submitted on November 20, 2001
Accepted on December 17, 2001

Production of {alpha}-1,3-Galactosyltransferase Knockout Pigs by Nuclear Transfer Cloning

Liangxue Lai 1, Donna Kolber-Simonds 2, Kwang-Wook Park 1, Hee-Tae Cheong 3, Julia L. Greenstein 2, Gi-Sun Im 4, Melissa Samuel 1, Aaron Bonk 1, August Rieke 1, Billy N. Day 1, Clifton N. Murphy 1, David B. Carter 5, Robert J. Hawley 2, Randall S. Prather 1*

1 Department of Animal Science, University of Missouri, Columbia, MO 65211, USA.
2 Immerge BioTherapeutics Inc., Charlestown, MA 02129, USA.
3 Department of Animal Science, University of Missouri, Columbia, MO 65211, USA; Department of Veterinary Medicine, College of Animal Resource Science, Kangwon National University, Chunchon 200-701, Korea.
4 Department of Animal Science, University of Missouri, Columbia, MO 65211, USA; National Livestock Research Institute, Suwon 441-350, Korea.
5 Department of Animal Science, Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA.

* To whom correspondence should be addressed. E-mail: pratherR{at}missouri.edu.

The presence of galactose {alpha}-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the {alpha}-1,3-galactosyltransferase locus has been knocked out. These pigs, were produced by nuclear transfer technology based on clonal fetal fibroblast cell lines as nuclear donors for embryos reconstructed with enucleated pig oocytes.


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