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Published Online January 24, 2002
Science DOI: 10.1126/science.1067861

Reports

Submitted on November 8, 2001
Accepted on January 8, 2002

Role of Nucleoporin Induction in Releasing an mRNA Nuclear Export Block

Jost Enninga 1, David E. Levy 2, Günter Blobel 1*, Beatriz M. A. Fontoura 3

1 Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA.
2 Department of Pathology and Kaplan Comprehensive Cancer Center, New York University School of Medicine, New York, New York 10016, USA.
3 Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA; Department of Molecular and Cellular Pharmacology and Sylvester Cancer Center, University of Miami School of Medicine, Miami, Florida 33136, USA.

* To whom correspondence should be addressed. E-mail: blobel{at}rockvax.rockefeller.edu.

Signal-mediated nuclear import and export proceeds through the nuclear pore complex (NPC). Some NPC components, such as the nucleoporins (Nups) Nup98 and Nup96, are also associated with the nuclear interior. Nup98 is a target of the vesicular stomatitis virus (VSV) matrix (M) protein-mediated inhibition of mRNA nuclear export. Here, Nup98 and Nup96 were found to be up-regulated by interferon (IFN). M protein-mediated inhibition of mRNA nuclear export was reversed when cells were treated with IFN{gamma} or transfected with a cDNA encoding Nup98 and Nup96. Thus, increased Nup98 and Nup96 expression constitutes an interferon-mediated mechanism that reverses M protein-mediated inhibition of gene expression.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)