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Published Online February 7, 2002
Science DOI: 10.1126/science.1067172

Reports

Submitted on October 16, 2001
Accepted on January 28, 2002

Protein Nanoarrays Generated By Dip-Pen Nanolithography

Ki-Bum Lee 1, So-Jung Park 1, Chad A. Mirkin 1*, Jennifer C. Smith 2, Milan Mrksich 2*

1 Northwestern University, Department of Chemistry and Center for Nanofabrication and Molecular Self-Assembly, 2145 Sheridan Road, Evanston, IL 60208, USA.
2 University of Chicago, Department of Chemistry and the Institute for Biophysical Dynamics, 5735 South Ellis, Chicago, IL 60637, USA.

* To whom correspondence should be addressed. E-mail: mmrksich{at}midway.uchicago.edu.

Dip-pen nanolithography (DPN) was used to construct arrays of proteins with 100 to 350 nanometer features. These nanoarrays exhibit almost no detectable nonspecific binding of proteins to their passivated portions even in complex mixtures of proteins, and therefore, provide the opportunity to study a variety of surface-mediated biological recognition processes. For example, reactions involving the protein features and antigens in complex solutions can be screened easily by atomic force microscopy (AFM). As further proof-of-concept, these arrays were used to study cellular adhesion at the submicrometer scale.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)