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Published Online January 17, 2002
Science DOI: 10.1126/science.1064913

Reports

Submitted on July 31, 2001
Accepted on January 3, 2002

Retrograde Support of Neuronal Survival Without Retrograde Transport of Nerve Growth Factor

Bronwyn L. MacInnis 1 Robert B. Campenot 1*

1 Department of Cell Biology, 6-14 Medical Sciences Building, University of Alberta, Edmonton, AB, Canada, T6G 2H7.

* To whom correspondence should be addressed. E-mail: bob.campenot{at}ualberta.ca.

Application of NGF covalently crosslinked to beads increased the phosphorylation of TrkA and Akt, but not MAPK in cultured rat sympathetic neurons. NGF beads or [125I] NGF beads supplied to distal axons resulted in the 30 h survival of over 80% of the neurons, with little or no retrograde transport of [125I] NGF, while application of 0.5 ng/ml free [125I] NGF produced 20-fold more retrograde transport, but only 29% of the neurons survived. Thus, in contrast to the widely-accepted theory, a neuronal survival signal can reach the cell bodies unaccompanied by the NGF that initiated it.


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