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Published Online April 19, 2001
Science DOI: 10.1126/science.1060360

Reports

Submitted on March 2, 2001
Accepted on April 9, 2001

A GDP/GTP Exchange Factor Involved in Linking a Spatial Landmark to Cell Polarity

Pil Jung Kang 1, Anthony Sanson 1, Bongyong Lee 1, Hay-Oak Park 1*

1 Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210-1292, USA.

* To whom correspondence should be addressed. E-mail: park.294{at}osu.edu.

In both animal and yeast cells, signaling pathways involving small GTPases regulate polarized organization of the actin cytoskeleton. In the budding yeast Saccharomyces cerevisiae, the Ras-like GTPase Bud1/Rsr1 and its GDP/GTP exchange factor (GEF) Bud5 are involved in selection of a specific site for growth, thus determining cell polarity. Here we showed that Bud5 was localized at the cell division site and the presumptive bud site. Its localization was dependent on potential cellular landmarks, such as Bud3 and Axl2/Bud10 in haploid cells, and Bud8 and Bud9 in diploid cells. Bud5 also physically interacted with Axl2/Bud10, a transmembrane glycoprotein, suggesting that a receptor-like transmembrane protein recruits a GEF to connect an intrinsic spatial signal to oriented cell growth.


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