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Published Online April 5, 2001
Science DOI: 10.1126/science.1059497

Reports

Submitted on February 1, 2001
Accepted on March 15, 2001

Regulation of Longevity and Stress Resistance by Sch9 in Yeast

Paola Fabrizio 1, Fabiola Pozza 1, Scott D. Pletcher 1, Christi M. Gendron 2, Valter D. Longo 1*

1 Division of Biogerontology, Andrus Gerontology Center, and Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089-0191, USA.
2 Max Planck Institute for Demographic Research, Rostock, D-18057 Germany.

* To whom correspondence should be addressed. E-mail: vlongo{at}usc.edu.

The protein kinase Akt/protein kinase B (PKB) is implicated in insulin signaling in mammals and functions in a pathway that regulates longevity and stress resistance in Caenorhabditis elegans. We screened for long-lived mutants in nondividing yeast Saccharomyces cerevisiae and identified mutations in adenylate cyclase and SCH9, which is homologous to Akt/PKB, that increase resistance to oxidants and extend life-span by up to threefold. Stress-resistance transcription factors Msn2/Msn4 and protein kinase Rim15 were required for this life-span extension. These results indicate that longevity is associated with increased investment in maintenance and show that highly conserved genes play similar roles in life-span regulation in S. cerevisiae and higher eukaryotes.


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