Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Published Online May 31, 2001
Science DOI: 10.1126/science.1057925

Reports

Submitted on November 30, 2000
Accepted on May 14, 2001

Stimulation of RNA Polymerase II Elongation by Hepatitis Delta Antigen

Yuki Yamaguchi 1, Julija Filipovska 2, Keiichi Yano 3, Akiko Furuya 3, Naoto Inukai 1, Takashi Narita 1, Tadashi Wada 1, Seiji Sugimoto 3, Maria M. Konarska 2, Hiroshi Handa 1*

1 Frontier Collaborative Research Center, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama 226-8503, Japan.
2 The Rockefeller University, New York, NY 10021, USA.
3 Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida, Tokyo 194-8533, Japan.

* To whom correspondence should be addressed. E-mail: hhanda{at}bio.titech.ac.jp.

Transcription elongation by RNA polymerase II (RNAPII) is negatively regulated by the human factors DRB-sensitivity inducing factor (DSIF) and negative elongation factor (NELF). A 66 kD subunit of NELF (NELF-A) shows limited sequence similarity to hepatitis delta antigen (HDAg), the viral protein required for replication of hepatitis delta virus (HDV). The host RNAPII has been implicated in HDV replication, but the detailed mechanism and the role of HDAg in this process are not understood. Here we show that HDAg binds RNAPII directly and stimulates transcription by displacing NELF and promoting RNAPII elongation. These results suggest that HDAg may regulate RNAPII elongation during both cellular mRNA synthesis and HDV RNA replication.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Combined proteomic-RNAi screen for host factors involved in human hepatitis delta virus replication.
D. Cao, D. Haussecker, Y. Huang, and M. A. Kay (2009)
RNA 15, 1971-1979
   Abstract »    Full Text »    PDF »
ERK1/2-Mediated Phosphorylation of Small Hepatitis Delta Antigen at Serine 177 Enhances Hepatitis Delta Virus Antigenomic RNA Replication.
Y.-S. Chen, W.-H. Huang, S.-Y. Hong, Y.-G. Tsay, and P.-J. Chen (2008)
J. Virol. 82, 9345-9358
   Abstract »    Full Text »    PDF »
Transcription of Subgenomic mRNA of Hepatitis Delta Virus Requires a Modified Hepatitis Delta Antigen That Is Distinct from Antigenomic RNA Synthesis.
C.-H. Tseng, K.-S. Jeng, and M. M. C. Lai (2008)
J. Virol. 82, 9409-9416
   Abstract »    Full Text »    PDF »
Formation of an RNA polymerase II preinitiation complex on an RNA promoter derived from the hepatitis delta virus RNA genome.
A. Abrahem and M. Pelchat (2008)
Nucleic Acids Res. 36, 5201-5211
   Abstract »    Full Text »    PDF »
Transcription Factor YY1 and Its Associated Acetyltransferases CBP and p300 Interact with Hepatitis Delta Antigens and Modulate Hepatitis Delta Virus RNA Replication.
W.-H. Huang, R.-T. Mai, and Y.-H. Wu Lee (2008)
J. Virol. 82, 7313-7324
   Abstract »    Full Text »    PDF »
Nucleolar Targeting of Hepatitis Delta Antigen Abolishes Its Ability To Initiate Viral Antigenomic RNA Replication.
W.-H. Huang, Y.-S. Chen, and P.-J. Chen (2008)
J. Virol. 82, 692-699
   Abstract »    Full Text »    PDF »
Wild-type is the optimal sequence of the HDV ribozyme under cotranscriptional conditions.
D. M. Chadalavada, A. L. Cerrone-Szakal, and P. C. Bevilacqua (2007)
RNA 13, 2189-2201
   Abstract »    Full Text »    PDF »
Hepatitis delta antigen binds to the clamp of RNA polymerase II and affects transcriptional fidelity.
Y. Yamaguchi, T. Mura, S. Chanarat, S. Okamoto, and H. Handa (2007)
Genes Cells 12, 863-875
   Abstract »    Full Text »    PDF »
Transcriptional Pausing Caused by NELF Plays a Dual Role in Regulating Immediate-Early Expression of the junB Gene..
M. Aida, Y. Chen, K. Nakajima, Y. Yamaguchi, T. Wada, and H. Handa (2006)
Mol. Cell. Biol. 26, 6094-6104
   Abstract »    Full Text »    PDF »
RNA-Templated Replication of Hepatitis Delta Virus: Genomic and Antigenomic RNAs Associate with Different Nuclear Bodies..
Y.-J. Li, T. Macnaughton, L. Gao, and M. M. C. Lai (2006)
J. Virol. 80, 6478-6486
   Abstract »    Full Text »    PDF »
Restoration in vivo of defective hepatitis delta virus RNA genomes.
S. O. Gudima, J. Chang, and J. M. Taylor (2006)
RNA 12, 1061-1073
   Abstract »    Full Text »    PDF »
Action of Inhibitors on Accumulation of Processed Hepatitis Delta Virus RNAs..
J. Chang, X. Nie, S. Gudima, and J. Taylor (2006)
J. Virol. 80, 3205-3214
   Abstract »    Full Text »    PDF »
RNA Replication without RNA-Dependent RNA Polymerase: Surprises from Hepatitis Delta Virus.
M. M. C. Lai (2005)
J. Virol. 79, 7951-7958
   Full Text »    PDF »
Molecular characterization of Drosophila NELF.
C.-H. Wu, C. Lee, R. Fan, M. J. Smith, Y. Yamaguchi, H. Handa, and D. S. Gilmour (2005)
Nucleic Acids Res. 33, 1269-1279
   Abstract »    Full Text »    PDF »
Reconstitution in cultured cells of replicating HDV RNA from pairs of less than full-length RNAs.
S. O. GUDIMA, J. CHANG, and J. M. TAYLOR (2005)
RNA 11, 90-98
   Abstract »    Full Text »    PDF »
Elongation by RNA polymerase II: the short and long of it.
R. J. Sims III, R. Belotserkovskaya, and D. Reinberg (2004)
Genes & Dev. 18, 2437-2468
   Abstract »    Full Text »    PDF »
{alpha}-Amanitin Blocks Translocation by Human RNA Polymerase II.
X. Q. Gong, Y. A. Nedialkov, and Z. F. Burton (2004)
J. Biol. Chem. 279, 27422-27427
   Abstract »    Full Text »    PDF »
Ser-123 of the large antigen of hepatitis delta virus modulates its cellular localization to the nucleolus, SC-35 speckles or the cytoplasm.
K.-P. Tan, K.-N. Shih, and S. J. Lo (2004)
J. Gen. Virol. 85, 1685-1694
   Abstract »    Full Text »    PDF »
Molecular Phylogenetic Analyses Indicate a Wide and Ancient Radiation of African Hepatitis Delta Virus, Suggesting a Deltavirus Genus of at Least Seven Major Clades.
N. Radjef, E. Gordien, V. Ivaniushina, E. Gault, P. Anais, T. Drugan, J.-C. Trinchet, D. Roulot, M. Tamby, M. C. Milinkovitch, et al. (2004)
J. Virol. 78, 2537-2544
   Abstract »    Full Text »    PDF »
Dynamics of Human Immunodeficiency Virus Transcription: P-TEFb Phosphorylates RD and Dissociates Negative Effectors from the Transactivation Response Element.
K. Fujinaga, D. Irwin, Y. Huang, R. Taube, T. Kurosu, and B. M. Peterlin (2004)
Mol. Cell. Biol. 24, 787-795
   Abstract »    Full Text »    PDF »
Combinatorial Control of Human RNA Polymerase II (RNAP II) Pausing and Transcript Cleavage by Transcription Factor IIF, Hepatitis {delta} Antigen, and Stimulatory Factor II.
C. Zhang, H. Yan, and Z. F. Burton (2003)
J. Biol. Chem. 278, 50101-50111
   Abstract »    Full Text »    PDF »
NTP-driven Translocation by Human RNA Polymerase II.
Y. A. Nedialkov, X. Q. Gong, S. L. Hovde, Y. Yamaguchi, H. Handa, J. H. Geiger, H. Yan, and Z. F. Burton (2003)
J. Biol. Chem. 278, 18303-18312
   Abstract »    Full Text »    PDF »
Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex.
T. Narita, Y. Yamaguchi, K. Yano, S. Sugimoto, S. Chanarat, T. Wada, D.-k. Kim, J. Hasegawa, M. Omori, N. Inukai, et al. (2003)
Mol. Cell. Biol. 23, 1863-1873
   Abstract »    Full Text »    PDF »
Dual Roles for Spt5 in Pre-mRNA Processing and Transcription Elongation Revealed by Identification of Spt5-Associated Proteins.
D. L. Lindstrom, S. L. Squazzo, N. Muster, T. A. Burckin, K. C. Wachter, C. A. Emigh, J. A. McCleery, J. R. Yates III, and G. A. Hartzog (2003)
Mol. Cell. Biol. 23, 1368-1378
   Abstract »    Full Text »    PDF »
C-terminal domain phosphatase-like family members (AtCPLs) differentially regulate Arabidopsis thaliana abiotic stress signaling, growth, and development.
H. Koiwa, A. W. Barb, L. Xiong, F. Li, M. G. McCully, B.-h. Lee, I. Sokolchik, J. Zhu, Z. Gong, M. Reddy, et al. (2002)
PNAS 99, 10893-10898
   Abstract »    Full Text »    PDF »
Evidence that Negative Elongation Factor Represses Transcription Elongation through Binding to a DRB Sensitivity-Inducing Factor/RNA Polymerase II Complex and RNA.
Y. Yamaguchi, N. Inukai, T. Narita, T. Wada, and H. Handa (2002)
Mol. Cell. Biol. 22, 2918-2927
   Abstract »    Full Text »    PDF »
Rolling Circle Replication of Hepatitis Delta Virus RNA Is Carried Out by Two Different Cellular RNA Polymerases.
T. B. Macnaughton, S. T. Shi, L. E. Modahl, and M. M. C. Lai (2002)
J. Virol. 76, 3920-3927
   Abstract »    Full Text »    PDF »
Host RNA Polymerase Requirements for Transcription of the Human Hepatitis Delta Virus Genome.
G. Moraleda and J. Taylor (2001)
J. Virol. 75, 10161-10169
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)