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Published Online February 1, 2001
Science DOI: 10.1126/science.1057269

Reports

Submitted on November 8, 2000
Accepted on January 22, 2001

Recovery of Infectious Ebola Virus from cDNA: Transcriptional RNA Editing of the GP Gene and Viral Cytotoxicity

Viktor E. Volchkov 1*, Valentina A. Volchkova 2, Elke Mühlberger 2, Larissa V. Kolesnikova 2, Michael Weik 2, Olga Dolnik 2, Hans-Dieter Klenk 2

1 University Claude Bernard Lyon-1, Filovirus Laboratory, 21 Avenue Tony Garnier, 69365 Lyon Cedex 07, France; Institut für Virologie, Philipps-Universität, Robert-Koch-Str. 17, 35037 Marburg, Germany.
2 Institut für Virologie, Philipps-Universität, Robert-Koch-Str. 17, 35037 Marburg, Germany.

* To whom correspondence should be addressed. E-mail: viktor.volchkov{at}ens-lyon.fr.

To study the mechanisms underlying the high pathogenicity of Ebola virus we have established a system that allows the recovery of infectious virus from cloned cDNA and, thus, genetic manipulating of the agent. We report here about a mutant in which the editing site of the glycoprotein gene was eliminated. This mutant did not longer express the non-structural glycoprotein sGP, whereas synthesis of the envelope glycoprotein GP was increased. However, most GP accumulated in the endoplasmic reticulum as immature precursor. The mutant was significantly more cytotoxic than wild type virus, indicating that cytotoxicity caused by GP expression is self-controlled by the virus through RNA editing and expression of sGP.


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