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Science 20 December 1996:
Vol. 274. no. 5295, pp. 2104 - 2107
DOI: 10.1126/science.274.5295.2104
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Reports

Associative Learning Disrupted by Impaired Gs Signaling in Drosophila Mushroom Bodies

John B. Connolly, * Ian J. H. Roberts, J. Douglas Armstrong, Kim Kaiser, Michael Forte, Tim Tully, Cahir J. O'Kane

Disruptions in mushroom body (MB) or central complex (CC) brain structures impair Drosophila associative olfactory learning. Perturbations in adenosine 3',5' monophosphate signaling also disrupt learning. To integrate these observations, expression of a constitutively activated stimulatory heterotrimeric guanosine triphosphate-binding protein alpha  subunit (Galpha s*) was targeted to these brain structures. The ability to associate odors with electroshock was abolished when Galpha s* was targeted to MB, but not CC, structures, whereas sensorimotor responses to these stimuli remained normal. Expression of Galpha s* did not affect gross MB morphology, and wild-type Galpha s expression did not affect learning. Thus, olfactory learning depends on regulated Gs signaling in Drosophila MBs.

J. B. Connolly, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA, and Department of Genetics, University of Cambridge, Cambridge, CB2 3EH, UK.
I. J. H. Roberts and C. J. O'Kane. Department of Genetics, University of Cambridge, Cambridge, CB2 3EH, UK.
J. D. Armstrong and K. Kaiser, Division of Molecular Genetics, Institute of Biomedical and Life Sciences, Pontecorvo Building, University of Glasgow, Glasgow, G11 6NU, UK.
M. Forte, Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, 3181 Southwestern Sam Jackson Park Road, Portland, OR 97201, USA.
T. Tully, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
*   To whom correspondence should be addressed. E-mail: connollj{at}cshl.org


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Metamorphosis of the Mushroom Bodies; Large-Scale Rearrangements of the Neural Substrates for Associative Learning and Memory in Drosophila.
J. D. Armstrong, J. S. de Belle, Z. Wang, and K. Kaiser (1998)
Learn. Mem. 5, 102-114
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Dopamine and Mushroom Bodies in Drosophila: Experience-Dependent and -Independent Aspects of Sexual Behavior.
W. S. Neckameyer (1998)
Learn. Mem. 5, 157-165
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Drosophila Mushroom Bodies Are Dispensable for Visual, Tactile, and Motor Learning.
R. Wolf, T. Wittig, L. Liu, G. Wustmann, D. Eyding, and M. Heisenberg (1998)
Learn. Mem. 5, 166-178
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Mushroom Bodies Suppress Locomotor Activity in Drosophila melanogaster.
J.-R. Martin, R. Ernst, and M. Heisenberg (1998)
Learn. Mem. 5, 179-191
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Defective Learning in Mutants of the Drosophila Gene for a Regulatory Subunit of cAMP-Dependent Protein Kinase.
S. F. Goodwin, M. Del Vecchio, K. Velinzon, C. Hogel, S. R. H. Russell, T. Tully, and K. Kaiser (1997)
J. Neurosci. 17, 8817-8827
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An activating mutation in a Caenorhabditis elegans Gs protein induces neural degeneration..
H C Korswagen, J H Park, Y Ohshima, and R H Plasterk (1997)
Genes & Dev. 11, 1493-1503
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A Return to Genetic Dissection of Memory in Drosophila.
T. Tully, G. Bolwig, J. Christensen, J. Connolly, M. DelVecchio, J. DeZazzo, J. Dubnau, C. Jones, S. Pinto, M. Regulski, et al. (1996)
Cold Spring Harb Symp Quant Biol 61, 207-218
   Abstract »    PDF »
The Role of Drosophila Mushroom Body Signaling in Olfactory Memory.
S. E. McGuire, P. T. Le, and R. L. Davis (2001)
Science 293, 1330-1333
   Abstract »    Full Text »    PDF »
Type II cAMP-dependent Protein Kinase-deficient Drosophila Are Viable but Show Developmental, Circadian, and Drug Response Phenotypes.
S. K. Park, S. A. Sedore, C. Cronmiller, and J. Hirsh (2000)
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