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Science 20 December 1996:
Vol. 274. no. 5295, pp. 2082 - 2086
DOI: 10.1126/science.274.5295.2082
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Reports

Intestinal Secretory Defects and Dwarfism in Mice Lacking cGMP-Dependent Protein Kinase II

Alexander Pfeifer, * Attila Aszódi, dagger Ursula Seidler, dagger Peter Ruth, Franz Hofmann, Reinhard Fässler

Cyclic guanosine 3',5'-monophosphate (cGMP)-dependent protein kinases (cGKs) mediate cellular signaling induced by nitric oxide and cGMP. Mice deficient in the type II cGK were resistant to Escherichia coli STa, an enterotoxin that stimulates cGMP accumulation and intestinal fluid secretion. The cGKII-deficient mice also developed dwarfism that was caused by a severe defect in endochondral ossification at the growth plates. These results indicate that cGKII plays a central role in diverse physiological processes.

A. Pfeifer, P. Ruth, F. Hofmann, Institut für Pharmakologie und Toxikologie, Technische Universität München, Biedersteiner Strasse 29, D-80802 München, Germany.
A. Aszódi and R. Fässler, Max-Planck-Institut für Biochemie, D-82152 Martinsried, Germany.
U. Seidler, Medizinische Klinik I, Eberhard-Karls-Universität Tübingen, D-72076 Tübingen, Germany.
*   To whom correspondence should be addressed. E-mail: pfeifer{at}ipt.med.tu-muenchen.de

dagger    These authors contributed equally to the work.


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   Abstract »    Full Text »    PDF »
Increased Adhesion and Aggregation of Platelets Lacking Cyclic Guanosine 3',5'-Monophosphate Kinase I.
S. Massberg, M. Sausbier, P. Klatt, M. Bauer, A. Pfeifer, W. Siess, R. Fassler, P. Ruth, F. Krombach, and F. Hofmann (1999)
J. Exp. Med. 189, 1255-1264
   Abstract »    Full Text »    PDF »
Long-Term Potentiation in the Hippocampal CA1 Region of Mice Lacking cGMP-Dependent Kinases Is Normal and Susceptible to Inhibition of Nitric Oxide Synthase.
T. Kleppisch, A. Pfeifer, P. Klatt, P. Ruth, A. Montkowski, R. Fassler, and F. Hofmann (1999)
J. Neurosci. 19, 48-55
   Abstract »    Full Text »    PDF »
Control of CFTR Channel Gating by Phosphorylation and Nucleotide Hydrolysis.
D. C. GADSBY and A. C. NAIRN (1999)
Physiol Rev 79, 77-107
   Abstract »    Full Text »    PDF »
Tissue Distribution, Cellular Source, and Structural Analysis of Rat Immunoreactive Uroguanylin.
M. Nakazato, H. Yamaguchi, Y. Date, M. Miyazato, K. Kangawa, M. F. Goy, N. Chino, and S. Matsukura (1998)
Endocrinology 139, 5247-5254
   Abstract »    Full Text »    PDF »
1,25-Dihydroxyvitamin D3 upregulates natriuretic peptide receptor-C expression in mouse osteoblasts.
N. Yanaka, H. Akatsuka, E. Kawai, and K. Omori (1998)
Am J Physiol Endocrinol Metab 275, E965-E973
   Abstract »    Full Text »    PDF »
Protein kinase G expression in the small intestine and functional importance for smooth muscle relaxation.
A. Huber, P. Trudrung, M. Storr, H. Franck, V. Schusdziarra, P. Ruth, and H.-D. Allescher (1998)
Am J Physiol Gastrointest Liver Physiol 275, G629-G637
   Abstract »    Full Text »    PDF »
Endogenous or overexpressed cGMP-dependent protein kinases inhibit cAMP-dependent renin release from rat isolated perfused kidney, microdissected glomeruli, and isolated juxtaglomerular cells.
S. Gambaryan, C. Wagner, A. Smolenski, U. Walter, W. Poller, W. Haase, A. Kurtz, and S. M. Lohmann (1998)
PNAS 95, 9003-9008
   Abstract »    Full Text »    PDF »
Natriuretic Peptide Regulation of Endochondral Ossification. EVIDENCE FOR POSSIBLE ROLES OF THE C-TYPE NATRIURETIC PEPTIDE/GUANYLYL CYCLASE-B PATHWAY.
A. Yasoda, Y. Ogawa, M. Suda, N. Tamura, K. Mori, Y. Sakuma, H. Chusho, K. Shiota, K. Tanaka, and K. Nakao (1998)
J. Biol. Chem. 273, 11695-11700
   Abstract »    Full Text »    PDF »
Skeletal overgrowth in transgenic mice that overexpress brain natriuretic peptide.
M. Suda, Y. Ogawa, K. Tanaka, N. Tamura, A. Yasoda, T. Takigawa, M. Uehira, H. Nishimoto, H. Itoh, Y. Saito, et al. (1998)
PNAS 95, 2337-2342
   Abstract »    Full Text »    PDF »
Membrane targeting of cGMP-dependent protein kinase is required for cystic fibrosis transmembrane conductance regulator Cl- channel activation.
Arie. B. Vaandrager, A. Smolenski, B. C. Tilly, A. B. Houtsmuller, E. M. E. Ehlert, A. G. M. Bot, M. Edixhoven, W. E. M. Boomaars, S. M. Lohmann, and H. R. de Jonge (1998)
PNAS 95, 1466-1471
   Abstract »    Full Text »    PDF »
Signaling Pathways for Guanylin and Uroguanylin in the Digestive, Renal, Central Nervous, Reproductive, and Lymphoid Systems.
X. Fan, Y. Wang, R. M. London, S. L. Eber, W. J. Krause, R. H. Freeman, and L. R. Forte (1997)
Endocrinology 138, 4636-4648
   Abstract »    Full Text »    PDF »
Hepatocyte nuclear factor-1alpha regulates transcription of the guanylin gene.
J. A. Hochman, D. Sciaky, T. L. Whitaker, J. A. Hawkins, and M. B. Cohen (1997)
Am J Physiol Gastrointest Liver Physiol 273, G833-G841
   Abstract »    Full Text »    PDF »
Natural Behavior Polymorphism Due to a cGMP-Dependent Protein Kinase of Drosophila.
K. A. Osborne, A. Robichon, E. Burgess, S. Butland, R. A. Shaw, A. Coulthard, H. S. Pereira, R. J. Greenspan, and M. B. Sokolowski (1997)
Science 277, 834-836
   Abstract »    Full Text »
Enhanced apoptotic cell death of renal epithelial cells in mice lacking transcription factor AP-2beta.
M. Moser, A. Pscherer, C. Roth, J. Becker, G. Mucher, K. Zerres, C. Dixkens, J. Weis, L. Guay-Woodford, R. Buettner, et al. (1997)
Genes & Dev. 11, 1938-1948
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Endogenous Type II cGMP-dependent Protein Kinase Exists as a Dimer in Membranes and Can Be Functionally Distinguished from the Type I Isoforms.
A. B. Vaandrager, M. Edixhoven, A. G. M. Bot, M. A. Kroos, T. Jarchau, S. Lohmann, H.-G. Genieser, and H. R. de Jonge (1997)
J. Biol. Chem. 272, 11816-11823
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The Amino-terminal Cyclic Nucleotide Binding Site of the Type II cGMP-dependent Protein Kinase Is Essential for Full Cyclic Nucleotide-dependent Activation.
M. K. Taylor and M. D. Uhler (2000)
J. Biol. Chem. 275, 28053-28062
   Abstract »    Full Text »    PDF »
Activation of Protein Kinase C Stimulates the Dephosphorylation of Natriuretic Peptide Receptor-B at a Single Serine Residue. A POSSIBLE MECHANISM OF HETEROLOGOUS DESENSITIZATION.
L. R. Potter and T. Hunter (2000)
J. Biol. Chem. 275, 31099-31106
   Abstract »    Full Text »    PDF »
Molecular Determinants of the Interaction between the Inositol 1,4,5-Trisphosphate Receptor-associated cGMP Kinase Substrate (IRAG) and cGMP Kinase Ibeta.
A. Ammendola, A. Geiselhoringer, F. Hofmann, and J. Schlossmann (2001)
J. Biol. Chem. 276, 24153-24159
   Abstract »    Full Text »    PDF »
Erectile dysfunction in cyclic GMP-dependent kinase I-deficient mice.
P. Hedlund, A. Aszodi, A. Pfeifer, P. Alm, F. Hofmann, M. Ahmad, R. Fassler, and K.-E. Andersson (2000)
PNAS 97, 2349-2354
   Abstract »    Full Text »    PDF »
Dwarfism and early death in mice lacking C-type natriuretic peptide.
H. Chusho, N. Tamura, Y. Ogawa, A. Yasoda, M. Suda, T. Miyazawa, K. Nakamura, K. Nakao, T. Kurihara, Y. Komatsu, et al. (2001)
PNAS 98, 4016-4021
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cGMP-Dependent Protein Kinase I Mediates the Negative Inotropic Effect of cGMP in the Murine Myocardium.
J. W. Wegener, H. Nawrath, W. Wolfsgruber, S. Kuhbandner, C. Werner, F. Hofmann, and R. Feil (2002)
Circ. Res. 90, 18-20
   Abstract »    Full Text »    PDF »


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