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Science 20 December 1996:
Vol. 274. no. 5295, pp. 2069 - 2074
DOI: 10.1126/science.274.5295.2069
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Reports

Functional Analysis of the Genes of Yeast Chromosome V by Genetic Footprinting

Victoria Smith, Karen N. Chou, Deval Lashkari, David Botstein, Patrick O. Brown *

Genetic footprinting was used to assess the phenotypic effects of Ty1 transposon insertions in 268 predicted genes of chromosome V of Saccharomyces cerevisiae. When seven selection protocols were used, Ty1 insertions in more than half the genes tested (157 of 268) were found to result in a detectable reduction in fitness. Results could not be obtained for fewer than 3 percent of the genes tested (7 of 268). Previously known mutant phenotypes were confirmed, and, for about 30 percent of the genes, new mutant phenotypes were identified.

V. Smith and K. N. Chou, Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA.
D. Lashkari and D. Botstein, Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
P. O. Brown, Howard Hughes Medical Institute and Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA.
*   To whom correspondence should be addressed. E-mail: pbrown{at}cmgm.stanford.edu


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