A Molecular Clutch Disables Flagella in the Bacillus subtilis Biofilm
Kris M. Blair, Linda Turner, Jared T. Winkelman, Howard C. Berg, Daniel B. Kearns

Supporting Online Material

This supplement contains:
Materials and Methods
SOM Text
Figs. S1 to S7
Tables S1 to S3
References

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Other Supporting Online Material for this manuscript includes the following: (available at www.sciencemag.org/cgi/content/full/320/5883/1636/DC1)
Movies S1 to S6

Movie s1
Cells that are uninduced for EpsE are vigorously motile. Phase contrast microscopy movie of DS2218 grown to mid log phase in LB broth.

Movie s2
The flagella of motile cells rapidly rotate. Fluorescence microscopy movie of DS2218 grown to mid-log phase in LB broth and stained with Alexa fluor 488 C5 maleimide prior to movie capture.

Movie s3
Cells induced for EpsE are non-motile. Phase contrast microscopy movie of DS2218 grown to mid-log phase in LB, induced for 40 minutes with 1 mM IPTG prior to movie capture.

Movie s4
EpsE inhibits flagellar rotation. Fluorescence microscopy movie of DS2218 grown to mid-log phase in LB, induced for 40 minutes with 1 mM IPTG, and stained with Alexa fluor 488 C5 maleimide prior to movie capture.

Movie s5
Wild type cells in multicellular aggregates are sessile. Phase contrast movie of wild type 3610 cells grown in shaken cultures of MSgg biofilm-promoting media at room temperature.

Movie s6
Cells that encode the "clutch-blind" FliG^V338A allele writhe in the confines of the biofilm matrix. Phase contrast movie of DS2959 sorE10 (fliG^V338A) cells grown in shaken cultures of MSgg biofilm-promoting media at room temperature.

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