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Science 18 April 2008:
Vol. 320. no. 5874, pp. 382 - 385
DOI: 10.1126/science.1155307


Divergence of Quaternary Structures Among Bacterial Flagellar Filaments
Vitold E. Galkin, Xiong Yu, Jakub Bielnicki, John Heuser, Cheryl P. Ewing, Patricia Guerry, Edward H. Egelman

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Materials and Methods
Fig. S1
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Movie S1

Movie s1
1) Cryo-EM images of the Campylobacter flagellar filaments were analyzed and sorted (using multi-reference cross-correlation against models having different symmetries). Starting with 21,913 initial segments, three subsets of filaments having a lefthanded coiling of the protofilaments were used for this animated power spectra. The twist of these subsets (defined by the rotation between subunits along the right-handed one-start helix) was: 102.2° (n=1,743); 102.5° (n=2,988); and 102.8° (n=2,914).

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Science. ISSN 0036-8075 (print), 1095-9203 (online)