Direct Visualization of Horizontal Gene Transfer
Ana Babić, Ariel B. Lindner, Marin Vulić, Eric Stewart, Miroslav Radman
Supporting Online Material
This supplement contains:
Materials and Methods
SOM Text
Figs. S1 to S4
References
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Other Supporting Online Material for this manuscript includes the following:
Movies S1 to S5
Movie s1
Real time conjugation between donors and recipients. A representative film of
conjugation between donors (ANB35, red cells) and recipients (ANB24, green cells) presented
as an overlay of red and green fluorescence images. The film shows 160 minutes (16 frames)
of the conjugation condensed to 15 seconds.
Movie s2
Transfer of the mrfp1 gene from donor to recipient. A representative film showing a
transfer of the mrfp1 gene from the donor (ANB35, red cells) to recipient (ANB24, green
cells), presented as an overlay of phase contrast and red and yellow fluorescent images. The
arrow points to the recipient where transfer of mrfp1 is observed. The film shows 220 minutes
(22 frames) of conjugation condensed to 21 seconds.
Movie s3
Conjugational transfer without visible cell contact between donor and recipient.
A representative film of conjugation without visible cell contact between donor (ANB35, red
cells) and recipient (ANB24, green cells), presented as an overlay of phase contrast and red and
yellow fluorescence images. The bars show the distance between recipient and the closest
donor. The film shows 110 minutes (11 frames) of conjugation condensed to 11 seconds.
Movie s4
Conjugation between donor and recombination deficient recipient.
A representative film of the degradation of a SeqA-YFP focus in the conjugation between
donor (ANB35, red cell) and a recombination deficient recipient (ANB26, green cell),
presented as an overlay of red and yellow fluorescent images. The film shows 80 minutes (8
frames) of conjugation condensed to 8 seconds.
Movie s5
Splitting of the SeqA-YFP focus. A representative film of a SeqA-YFP focus
splitting in a recipient (ANB24) cell during post-conjugation incubation, presented as an
overlay of phase contrast and yellow fluorescent images. The film shows 300 minutes (20
frames) condensed to 5 seconds.
To view these movies, download a QuickTime viewer.
