Evidence for Karyogamy and Exchange of Genetic Material in the
Binucleate Intestinal Parasite Giardia intestinalis
Marianne K. Poxleitner, Meredith L. Carpenter, Joel J. Mancuso, Chung-Ju R. Wang,
Scott C. Dawson, W. Zacheus Cande
Supporting Online Material
This supplement contains:
Materials and Methods
Figs. S1 and S2
References
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Correction: 19 March 2008
The source of an unpublished protocol was mistakenly omitted from the
"Encystation" section of the Materials and Methods.
The original version can be found
here
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Other Supporting Online Material for this manuscript includes the following:
(available at www.sciencemag.org/cgi/content/full/319/5869/1530/DC1)
Movies S1 to S6
Movie s1
Nuclear movements in a live, acridine orange-stained cyst.
Frames were taken every second for 1 minute.
Movie s2
Nuclei are immobile in trophozoites. Acridine orange-stained
trophozoites, which are attached to the slide via their ventral discs, move continuously by
beating their flagella. The nuclei are firmly attached to the cytoskeleton and remain
stationary. Frames were taken every second for 30 seconds.
Movie s3
Nuclear movements in a live, acridine orange-stained cyst.
Frames were taken every 0.5 seconds for 2 minutes.
Movie s4
Immunofluorescence images from Figure 3 A-C viewed as
three-dimensional projections. Movie 4 shows a cyst with four nuclei (red) at opposite
ends of the cell, while Movie 5 shows a cyst with four nuclei together at one end of cell,
exhibiting no nuclear fusion, and Movie 6 shows a cyst with three nuclei.
Movie s5
Immunofluorescence images from Figure 3 A-C viewed as
three-dimensional projections. Movie 4 shows a cyst with four nuclei (red) at opposite
ends of the cell, while Movie 5 shows a cyst with four nuclei together at one end of cell,
exhibiting no nuclear fusion, and Movie 6 shows a cyst with three nuclei.
Movie s6
Immunofluorescence images from Figure 3 A-C viewed as
three-dimensional projections. Movie 4 shows a cyst with four nuclei (red) at opposite
ends of the cell, while Movie 5 shows a cyst with four nuclei together at one end of cell,
exhibiting no nuclear fusion, and Movie 6 shows a cyst with three nuclei.
To view these movies, download a QuickTime viewer.
