In Situ Imaging of the Endogenous CD8 T Cell Response to Infection
Kamal M. Khanna, Jeffery T. McNamara, Leo Lefrançois
Supporting Online Material
This supplement contains:
Methods
Figures S1 to S12
References
Download supplement
This file is in Adobe Acrobat PDF format.
Other Supporting Online Material for this manuscript includes the following:
(available at www.sciencemag.org/cgi/content/full/318/5847/116/DC1)
Movies S1 to S3
Movie s1
Movie showing a magnified view of a tet+ CD8 T cell in direct contact with a CD11c+ DC. Note the polarized OVA-specific TCR on the CD8 T cell. The image shown in Fig. 1D was reconstituted and the IsoSurface module of Imaris was applied to the data in the blue (representing the CD8 stain), red (OVA tetramer) and green (CD11c) channel to generate a magnified 3D reconstructed image. The original image was acquired using a 40x 1.3-NA water objective.
Movie s2
Antigen-specific CD8 T cells exit the white pulp via the bridging channels. Sections of spleen (5 days PI) were stained for blood vessels (CD31-specific antibody; green) to identify the central arteriole and associated branches (also see Fig. S6) and B220-specific antibody (green). The movie shows a rendered 3D reconstruction of the boxed region in Fig. 1B (also shown in the right most (4th) panel of bottom row of images of Fig. 1B) illustrating an exit point (bridging channel) for OVA-Tet+ (red) cells.
Movie s3
Memory cells are embedded in B cell follicles. A 3D reconstruction of a merged z stack shows OVA-tet+ (red) CD8+ (blue) T cells embedded in the B cell (green) follicle.
To view these movies, download a QuickTime viewer.
