Differential Transmission of Actin Motion Within Focal Adhesions
Ke Hu, Lin Ji, Kathryn T. Applegate, Gaudenz Danuser, Clare M. Waterman-Storer

Supporting Online Material

This supplement contains:
Materials and Methods
Figs. S1 to S8
Tables S1 to S5
References
Movies S1 to S14

This file is in Adobe Acrobat PDF format.

Supplementary movie 1.
TIR-FSM time-lapse movie of _v-₃ integrin-GFP.

Supplementary movie 2.
TIR-FSM time-lapse movies of three FA core proteins tagged with GFP: FAK, zyxin and paxillin.

Supplementary movie 3.
TIR-FSM time-lapse movies of three FA actin binding proteins tagged with GFP: vinculin, talin and -actinin.

Supplementary movie 4.
TIR-FSM time-lapse movies of GFP-actin and Alexa568-actin. Note that some Alexa568 actin sticks to the coverslip surface during the microinjection procedure.

Supplementary movie 5.
Dual-color TIR-FSM time-lapse imaging of _v-₃-integrin-GFP and X-rhodamine-actin and the color coded DCS map.

Supplementary movie 6.
Dual-color TIR-FSM time-lapse imaging of GFP-FAK and Alexa568-actin and the color coded DCS map.

Supplementary movie 7.
Dual-color TIR-FSM time-lapse imaging of GFP-zyxin and X-rhodamine-actin and the color coded DCS map.

Supplementary movie 8.
Dual-color TIR-FSM time-lapse imaging of GFP-paxillin and X-rhodamine-actin and the color coded DCS map.

Supplementary movie 9.
Dual-color TIR-FSM time-lapse imaging of GFP-vinculin and X-rhodamine-actin and the color coded DCS map.

Supplementary movie 10.
Dual-color TIR-FSM time-lapse imaging of GFP-talin and X-rhodamine-actin and the color coded DCS map.

Supplementary movie 11.
Dual-color TIR-FSM time-lapse imaging of GFP--actinin and X-rhodamine-actin and the color coded DCS map.

Supplementary movie 12.
Top row: TIR-FSM time-lapse imaging of GFP-vinculin (green) and X-rhodamine-actin (red) and the color coded DCS map. Bottom row: color-coded speed map of vinculin (left), F-actin (middle) and the cell-edge trace (right).

Supplementary movie 13.
Movies generated by Monte Carlo simulation of fluorescent FA proteins diffusing at the rate shown while bound to an immobile FA platform and uncoupled from a flowing F-actin network (not shown) (diffusion constant D in cm²/s).

Supplementary movie 14.
Movies generated by Monte Carlo simulation to show three levels of coupling (increasing left to right) between fluorescent FA proteins (green) and the F-actin network (red).

To view these movies, download a QuickTime viewer.