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Science 3 November 2006:
Vol. 314. no. 5800, pp. 818 - 821
DOI: 10.1126/science.1134027


Protrudin Induces Neurite Formation by Directional Membrane Trafficking
Michiko Shirane and Keiichi I. Nakayama

Supporting Online Material

This supplement contains:
Materials and Methods
Figs. S1 to S8
Movies S1 and S2

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Movie S1
Time-lapse analysis of process formation induced by overexpression of protrudin. HeLa cells were transfected with an expression plasmid for EGFP-tagged protrudin, and EGFP fluorescence was monitored by video microscopy. Images of cells obtained from 12 to 30 hours after transfection were recorded at intervals of 5 min.

Movie S2
Time-lapse analysis of cells expressing ectopic protrudin and Rab11(Q70L). HeLa cells were transfected with expression plasmids for EGFP-tagged protrudin and DsRed-tagged Rab11(Q70L), and EGFP fluorescence and DIC images were recorded by video microscopy.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)